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通过改变SOCS1水平,SARS-CoV2感染诱导miR-155表达和歪曲Th17/Treg平衡:一项临床研究

Mohammad Sadegh Soltani-Zangbar¹, Mahsa Hajivalili², Danyal Daneshdoust³

¹Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

|June 12, 2023

概括

微RNA-155 (miR-155) 在COVID-19患者中升级,影响T辅助17/调控T细胞平衡. MiR-155可以作为COVID-19严重程度的诊断和预后标志物.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
病毒学病毒学

背景情况:

微RNAs (miRNAs) 在SARS-CoV2感染期间起到调节免疫反应的作用.
miR-155是一种与炎症相关的miRNA,可能会影响COVID-19患者的免疫反应.

研究的目的:

研究miR-155在COVID-19中的作用.
在COVID-19患者中分析miR-155,T助手17 (Th17) 细胞,调节性T (Treg) 细胞和相关信号分子之间的关系.

主要方法:

分析了来自50名COVID-19患者和健康对照的外周血液单核细胞 (PBMC).
使用流式细胞计,实时PCR,西部抹杀和ELISA来评估细胞群,基因/蛋白质表达 (miR-155,SOCS-1,STAT3,FoxP3,RORγT) 和血清细胞因子水平 (IL-10,TGF-β,IL-17,IL-21).

主要成果:

COVID-19患者的Th17细胞增加,Treg细胞减少,STAT3表达 (RNA和蛋白质) 增加.
miR-155在COVID-19 PBMC中被上调,与SOCS-1负相关.
在COVID-19病例中,血清分析显示TGF-β降低,IL-17,IL-21和IL-10水平增加.

结论:

miR-155可能会影响COVID-19中的Th17/Treg平衡.
miR-155可能是COVID-19的潜在诊断和预后生物标志物.

关键词:

细胞免疫力细胞免疫力微型RNA微型RNA是什么一个T细胞细胞.病毒感染是一种病毒感染.

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