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准备和评估基于Trueperella pyogenes的复合型皮奥利辛,Fimbriae E和HtaA蛋白质疫苗,以预防Trueperella pyogenes的感染

Yongsheng Cao¹, Yunlu Bai¹, Hanqing Li¹

¹Laboratory of Veterinary Immunology, Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Changjiang Street NO.600, Harbin, China; Northeastern Science Inspection Station, China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Harbin, China.

Veterinary microbiology

|June 12, 2023

概括

使用Trueperella pyogenes (T. pyogenes) 成分的新型蛋白质疫苗,如pyolysin突变 (PLOW497F) 和HtaA-2,显示出有前途. 这些疫苗显著保护了小鼠免受致命的T. pyogenes挑战,表明有可能防止动物传播疾病造成的经济损失.

科学领域:

兽医免疫学兽医免疫学
细菌学细菌学是一门学科.
疫苗开发疫苗开发

背景情况:

特鲁佩雷拉 pyogenes (T. pyogenes) 在哺乳动物中引起动物性疾病,导致经济损失很大.
现有的疫苗无效,细菌耐药性正在上升,需要新的疫苗策略.
关键的T. pyogenes病毒性因素包括pyolysin (PLO),fimbriae E (FimE) 和细胞壁蛋白HtaA.

研究的目的:

开发和评估针对T. pyogenes的单种或多种蛋白质疫苗.
在小鼠模型中评估非溶血型皮奥利辛突变体 (PLOW497F),FimE和HtaA-2的疗效.

主要方法:

使用重组PLOW497F,FimE和HtaA-2生成单种和多种蛋白质疫苗.
在小鼠免疫接种之后,致命的T. pyogenes挑战.
测量特异性抗体水平,细胞因子基因表达,抗血解和凝结性抗体标位,以及病理性病变.

主要成果:

与对照群相比,接种疫苗的小鼠在补充疫苗接种后表现出明显更高的特定抗体水平.
单独或组合使用PLOW497F和HtaA-2可以完全保护人免受致命的T. pyogenes挑战.
联合免疫增强了抗血解抗体,而HtaA-2则比单抗原更有效地增强了聚合抗体.

结论:

重组PLOW497F和HtaA-2是有效的疫苗候选人,可以预防T. pyogenes感染.
这些蛋白质显示出开发有效疫苗的潜力,以减轻T. pyogenes zoonoses造成的经济损失.
结合这些抗原的多价值疫苗可能会提供更强的保护.

关键词:

免疫特性免疫性质保护保护保护保护亚单位疫苗亚单位疫苗真贝拉 (Trueperella pyogenes) 是一种天然的植物.病毒性因素是病毒性因素.

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