人类胚胎囊中的可转移元素的组成动态
Jian Li1, Ping Yuan2,3, Guangwei Ma4
1Department of Clinical Laboratory, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.
Journal of human genetics
|June 12, 2023
概括
人类胚芽细胞中可移植元素 (TE) 的比例受到父母遗传背景和胚胎发育阶段的显著影响. 这些移动DNA序列在早期发育过程中显示出动态变化.
科学领域:
- 遗传学 遗传学 是一个
- 发展生物学 发展生物学
- 基因组学就是基因组学.
背景情况:
- 可转移元素 (TE) 是移动DNA序列,对基因组进化和调节至关重要.
- 在胚胎发育和染色体结构中,TEs起作用,但它们在人体胚胎囊中的变异尚未完全理解.
研究的目的:
- 根据父母的遗传背景和发育阶段,研究人类胚芽细胞中可移植元素 (TE) 的变异.
- 为了确定特定的TE子家族,受父母的型,胚胎囊阶段和状况的影响.
主要方法:
- 在196个胚胎囊中,在DNA层面上对6个类别的1137个TE子家族进行了分析.
- 使用Bowtie2和PopoolationTE2进行TE比例分析.
- 相关的TE频率与父母的型,胚胎细胞的发育阶段,和 ploidy 状态.
主要成果:
- 家长的型是影响TE频率的主要因素,有1116个子家族显示变化.
- 胚芽细胞发育阶段是影响614个子家族的第二个最关键因素.
- 特定的TE家族,如Alu和LINE在不同胚胎细胞阶段 (例如第6阶段和第3阶段) 显示出不同的比例.
- TE亚家族的比例也因胚胎细胞核型 (平衡或不平衡),内细胞质量和外层皮状况而有所不同.
结论:
- 在人类胚胎发育过程中,TE亚家族的组成是动态调节的.
- 父母遗传背景和发育阶段显著塑造了胚胎细胞中TE景观.
- TE变异可以作为胚胎健康和发育潜力的指标.
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