替代的Z基因组生物合成途径显示了从Archaea到菌体的进化进展
Yang Tong1,2,3,4, Xinying Wu5,6, Yang Liu7,8
1Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, Collaborative Innovation Center of Chemical Science and Engineering, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China.
Nature microbiology
|June 12, 2023
概括
菌体使用2,6-二氨基氨酸 (Z) 来逃避免疫系统. 一种新发现的变异,PurZ0,为酶如何从使用ATP适应GTP提供了进化见解,为Z基因组生命铺平了道路.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 进化生物学 进化生物学
背景情况:
- 菌体在它们的基因组中使用2,6-氨酸 (Z) 而不是腺素,以逃避细菌的免疫防御.
- Z-核酸的生物合成涉及PurZ酶,它与腺酸合成酶 (PurA) 有关,并且与古老的PurA酶有相似之处.
研究的目的:
- 研究PurA向PurZ的进化过渡,并了解Z基因组菌体的起源.
- 识别和描述PurA-PurZ进化路径中的中间形式.
主要方法:
- 通过计算机指导识别自然存在的PurZ变体.
- 鉴定到的PurZ变种的生物化学特征,PurZ0.
- 对PurZ0.0的原子分辨率结构分析.
- 遗传学分析以推断进化关系.
主要成果:
- 确定了一种自然存在的PurZ变体,PurZ0.
- PurZ0使用三酸盐 (GTP) 作为酸盐供体,与正规的PurZ.0使用的ATP不同.
- 结构分析显示,PurZ0具有与考古PurA.类似的关氨酸核酸结合口袋.
- 遗传学分析将PurZ0定位为古老PurA和菌体PurZ之间的潜在进化中间体.
结论:
- PurZ0代表了从古老的PuraA到菌素PurZ的进化途径中的关键中间体.
- 通过PurZ0从GTP转变为ATP利用,对于适应Z基因组生命和维持 purin平衡至关重要.
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