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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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多波长多方向激光光散射用于细胞表征,使用基于机器学习的方法.

Lina Liu1, Md Zahurul Islam1,2, Xiaoxuan Liu1

  • 1Department of Electrical and Computer Engineering, University of Alberta, Edmonton, Canada.

Cytometry. Part A : the journal of the International Society for Analytical Cytology
|June 13, 2023
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概括
此摘要是机器生成的。

角激光光散射模式 (ALSP) 提供无标签的细胞分析. 倒向散射最能揭示表面粗度,而前向散射则能使线粒体区分开来,红色或绿色光的效果优于蓝色光.

关键词:
角光散射是一种角光散射.没有标签的细胞计量.机器学习是机器学习.数值光散射模拟的数值光散射模拟一个单细胞分析.

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科学领域:

  • 生物物理学的生物物理.
  • 细胞生物学 细胞生物学
  • 光学物理学的光学物理学

背景情况:

  • 细胞识别对于生物学和健康应用至关重要.
  • 光学显微镜与亚微米细胞特征作斗争.
  • 角激光光散射模式 (ALSP) 显示出无标签细胞分析的前景.

研究的目的:

  • 使用ALSP.研究细胞表面粗度和线粒体数量.
  • 确定最佳的激光波长和分散收集方向,以实现细胞特性差异化.
  • 评估激光波长对基于ALSP的细胞信息的影响.

主要方法:

  • 单细胞ALSP的数值模拟.
  • 使用机器学习 (ML) 分析ALSP.
  • 蓝色,绿色和红色激光波长的系统研究.
  • 对前方,侧面和后方散射光收集的检查.

主要成果:

  • 逆向散射是表征细胞表面粗度的最佳方法.
  • 前向散射是对分化线粒体数量的最佳方法.
  • 红色和绿色激光波长在区分这些细胞特性方面比蓝色更有效.

结论:

  • ALSP,结合ML,可以有效地区分细胞特性.
  • 散射方向和激光波长显著影响ALSP的信息检索.
  • 这项研究为优化细胞分析中的ALSP技术提供了关键的见解.