由赫xabromocyclododecane (HBCD) 诱导的细胞死亡机制包括亡,自和ER压力
Noor A Mohammed1,2, Kirstie Lewis3, Nikolas Hodges1
1School of Biosciences, University of Birmingham, Edgbaston, Birmingham, UK.
Journal of biochemical and molecular toxicology
|June 13, 2023
概括
赫克萨布罗摩环多德干 (HBCD) 对神经和免疫细胞有毒,比肝脏和细胞更有毒. HBCD通过,亡和自诱导细胞死亡,可能通过ER压力,无论细胞类型如何.
科学领域:
- 环境毒理学环境毒理学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 赫克萨布罗摩环多德 (HBCD) 是一种常见的制阻燃剂,在消费品中发现.
- 在食物和人体组织中存在的HBCD引起了人们对其潜在的健康影响的担忧.
- 之前的研究表明,HBCD是一种令人担忧的化学物质,因为它在环境中存在广泛的持久性.
研究的目的:
- 为了评估来自不同组织的各种细胞系中HBCD的细胞毒性.
- 为了确定HBCD是否对特定细胞类型表现出差异性毒性影响.
- 阐明HBCD诱导的细胞死亡的潜在机制.
主要方法:
- 对白细胞,神经元,肝脏和脏衍生的细胞系进行了细胞毒性测试.
- 在每个细胞系中确定了HBCD的半最大致死度 (LC50) 值.
- 机械学研究研究了依赖的细胞死亡,细胞亡,自,亡,亡和ER应激反应.
主要成果:
- 与肝 (HepG2) 和 (Cos-7) 细胞相比,HBCD对白细胞 (RBL2H3) 和神经元 (SHSY-5Y) 细胞的毒性显著更高.
- 由HBCD诱导的细胞死亡的主要机制包括Ca2+依赖的途径,酶激活的亡和自.
- 细胞内膜网膜 (ER) 压力被确定为亡和自的潜在触发因素,这表明它在HBCD的作用模式中起着关键作用.
结论:
- HBCD表现出细胞类型特定的毒性,对神经元和免疫细胞更有效.
- HBCD通过亡,自和依赖的途径的组合诱导细胞死亡,这可能是由ER压力引起的.
- 由HBCD诱导的细胞死亡机制在不同细胞类型中看起来是一致的,这表明一种保存的作用模式.
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