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Updated: Jun 20, 2026

High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii
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High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii

Published on: December 5, 2016

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用于多种类型的精确基因编辑的高效方法在Chlamydomonas中

Hui Chen1, Qing-Lin Yang1, Jia-Xi Xu1,2

  • 1State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, 200031, China.

The Plant journal : for cell and molecular biology
|June 13, 2023
PubMed
概括

这项研究引入了Clamydomonas reinhardtii的精确CRISPR/Cas9基因编辑,使基因失活,标记和精确修改成为可能. 这些进步增强了这个模型生物在研究和工业中的实用性.

关键词:
克拉米多莫纳斯病毒是什么?有效的高效的高效.基因编辑是一种基因编辑.通过同质介导的同质介导.多种类型的多种类型.精确的精确度可以说是精确的.

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Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...