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通过干细胞移植生产人性化的小鼠

Cameron Phelps¹, Devra D Huey¹, Stefan Niewiesk¹

¹Department of Veterinary Biosciences, The Ohio State University, Columbus, Ohio.

Current protocols

|June 13, 2023

概括

用免疫缺陷小鼠中使用人类带干细胞制造的人类化小鼠,对于研究人类疾病非常有价值. 在NSG背景下生成和分析这些人性化小鼠的协议是详细的.

科学领域:

免疫学免疫学免疫学
翻译医学是一种翻译医学.
动物模型动物模型

背景情况:

人性化小鼠对于研究人体疾病至关重要.
严重免疫缺陷的小鼠菌株对于成功移植人类细胞至关重要.
人类带干细胞是创建人性化模型的关键组成部分.

研究的目的:

介绍已建立的产生人性化小鼠的协议.
详细介绍分析人性化小鼠模型的方法.
为使用NSG鼠标背景的研究人员提供指南.

主要方法:

将人类带干细胞植入新生儿和4周大的免疫缺陷小鼠.
用于移植的人类带干细胞的准备.
在人性化小鼠中通过在下下下收集血液后的流动细胞计对外周围血液的分析.

主要成果:

在免疫缺陷小鼠中成功移植和开发人类淋巴细胞.
使用NSG背景证明了人性化小鼠生成的可行性.
采样和分析的既定方法.

结论:

提出的协议使得人类化小鼠的可靠生成和分析成为可能.
人性化小鼠是人类疾病转化研究的强大平台.
这些方法有助于研究人体免疫系统的发育和 in vivo 的功能.

关键词:

NOD.SCID 玛 (NSG) 人性化的小鼠人类免疫系统 (HIS)人类白细胞抗原A2 (HLA-A2)人类带干细胞 (HUSCs)

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