急性动脉损伤中的巨细胞激活和脱粒化:手术后治疗的目标
Rebecca L Harper1, Fang Fang1, Hong San1
1Translational Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA.
概括
乳腺细胞迅速对动脉损伤作出反应,导致炎症和新极端增生. 用二克罗莫糖酸向瘤细胞的向可能会在PCI等手术后预防复原.
科学领域:
- 心血管研究研究心血管研究
- 免疫学 免疫学 免疫学
- 血管生物学 血管生物学
背景情况:
- 心血管疾病 (CVD) 需要像冠状动脉旁路移植 (CABG) 和皮肤冠状动脉干预 (PCI) 这样的手术干预.
- 这些手术后的内皮损伤导致静脉缩,对死亡率和发病率做出了重大贡献.
- 乳腺细胞 (MC) 与血管疾病有关,但它们在动脉损伤反应中的急性作用尚未完全理解.
研究的目的:
- 调查巨细胞 (MC) 在对动脉线损伤的急性反应中的作用,模仿穿皮冠状动脉干预 (PCI).
- 评估MC对新极度增生症和相关的炎症细胞透的影响.
- 评估针对性MC脱粒的治疗潜力,以预防受伤后复原.
主要方法:
- 使用野生类型和缺少乳腺细胞 (KitW-sh/W-sh) 的小鼠遭受大腿动脉电线损伤.
- 分析了受伤后的巨细胞积累,激活和脱粒.
- 量化了新极端增生和炎症细胞 (中性粒细胞,巨细胞,T细胞) 的存在.
- 研究了骨髓衍生性瘤细胞 (BMMC) 移植的效果.
- 管理的二染糖酸盐 (DSCG),一个巨细胞稳定剂,受伤后.
主要成果:
- 乳腺细胞迅速积累,激活,并在野生类型小鼠的股骨动脉中脱粒,在电线受伤后.
- 在野生类型小鼠中观察到显著的新极限增生症,但在乳腺细胞缺乏的小鼠中没有.
- 在野生型小鼠中,中性粒细胞,巨细胞和T细胞在受伤部位丰富,在缺乏细胞的小鼠中存在较少.
- 将BMMC移植到缺陷小鼠中恢复了neointimal增生和炎症细胞透.
- 两血糖酸盐 (DSCG) 的使用显著降低了野生类型小鼠的新极端增生症.
结论:
- 乳腺细胞在急性动脉损伤后的炎症反应和新极端增生症中发挥着关键作用.
- 肌肉内膜脱粒是内皮损伤后有害细胞反应的关键驱动因素.
- 在手术后立即使用酸糖酸盐 (DSCG) 等药物向瘤细胞脱粒化,这为预防重血管化手术中的复缩提供了一个潜在的策略.
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