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高通量SARS-CoV-2抗病毒检测方法使用Celigo图像细胞计

Laura A St Clair1,2, Leo Li-Ying Chan3, Adam Boretsky4

  • 1Center for Vector-borne Infectious Diseases, Department of Microbiology, Immunology, and Pathology, Colorado State University, CO, 80523, Fort Collins, USA.

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概括

研究人员使用Celigo图像细胞计开发了一种快速,高通量抗病毒测试方法. 这种新方法显著减少了测试时间,以确定有效的SARS-CoV-2抗病毒药物对新出现的变种.

关键词:
抗病毒药物是一种抗病毒药.在 COVID-19 疫情中,在Celigo的世界里.药物重新定位是药物重新定位.图像细胞计量 图像细胞计量这就是SARS-CoV-2病毒.

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科学领域:

  • 病毒学 病毒学
  • 药物发现 药物发现 药物发现
  • 生物技术是生物技术.

背景情况:

  • 由于COVID-19大流行导致全球卫生危机,迫使疫苗和抗病毒药物的快速开发.
  • 新出现的SARS-CoV-2变种令人担忧,需要不断发现具有高疗效的新抗病毒药物候选者.
  • 传统的抗病毒检测方法,如斑块减少中和测试 (PRNTs) 和斑块测试,耗时且劳动密集.

研究的目的:

  • 使用Celigo图像细胞计开发一种高通量SARS-CoV-2抗病毒检测方法.
  • 评估抗病毒药物候选剂对SARS-CoV-2感染性的疗效及其对宿主细胞的安全性.
  • 与传统方法相比,减少抗病毒药物查的处理时间.

主要方法:

  • 利用光记者病毒来监测SARS-CoV-2的感染性.
  • 使用Celigo图像细胞计进行抗病毒药物候选者的高通量查.
  • 在人类细胞系上使用光活力染色剂评估候选药物的细胞毒性.

主要成果:

  • 开发的方法显著减少了平均3-4天的抗病毒测试时间.
  • 成功地直接利用人类细胞系,这些细胞系通常不适合PRNT或斑块检测.
  • 证明了Celigo图像细胞计对高效和强大的抗病毒查的能力.

结论:

  • 凯利戈图像细胞计为快速发现针对SARS-CoV-2及其变种的抗病毒药物提供了一个高效和强大的平台.
  • 这种高通量方法加快了在正在进行的流行病期间需要的潜在抗病毒药物的识别.
  • 该方法允许对相关的人类细胞系进行直接测试,提高抗病毒查的生物相关性.