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在两个压力因素下,Pseudochlorella pringsheimii的抗氧化酶:SOD异型的活性变化

Mostafa M S Ismaiel¹, Michele D Piercey-Normore²

¹Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt. mostafamsami@yahoo.com.

Journal of plant research

|June 13, 2023

概括

这项研究表明,铁和盐分压力会影响Pseudochlorella pringsheimii的生长和抗氧化酶活性. 铁在低剂量时增加了细胞数量,而这两种压力都增加了超氧化物脱酶 (SOD),酶 (CAT) 和过氧化酶 (POD) 的活性.

科学领域:

*藻类生理学和应激反应
* 生物化学和酶动力学
* 环境生物技术

背景情况:

*藻类面临重大环境压力,需要适应性生存机制.
*了解微藻如Pseudochlorella pringsheimii的应激耐受性对于各种应用至关重要.
*抗氧化酶在减轻环境压力因素引起的细胞损伤方面发挥着关键作用.

研究的目的:

* 为了研究铁和盐分压力对Pseudochlorella pringsheimii的生长的影响.
* 分析抗氧化酶的活性和表达,包括超氧化脱氧化酶 (SOD) 异型,催化酶 (CAT) 和过氧化酶 (POD).
* 确定酶活性,基因表达和压力水平之间的相关性.

主要方法:

* 在不同度的铁 (Fe) 和化 (NaCl) 下种植Pseudochlorella pringsheimii.
*测量藻类细胞数量和定量抗氧化酶活性 (SOD,CAT,POD).
*使用凝电泳和体外试验分析SOD异型 (Mn,Fe,Cu/Zn SOD) 和FeSOD相对基因表达的分析.

主要成果:

*0.025-0.09毫米的铁适度增加细胞数量;更高的度和所有测试的NaCl水平抑制了生长.
* 超氧化物脱酶 (SOD) 活性,特别是FeSOD,与铁显著增加,与盐度不显著增加.
* 在铁和盐度压力下,酶 (CAT) 和过氧化酶 (POD) 活动加速,在FeSOD活性和表达方面观察到高正相关性.

结论:

* Pseudochlorella pringsheimii对铁和度压力表现出不同的生长反应.
* 铁和盐度诱导显著的抗氧化酶活性,突出显示它们在应激适应中的作用.
*FeSOD是这种藻类应激反应中的关键酶,其表达水平与应激条件相关.

关键词:

抗氧化酶酶是一种抗氧化酶.叶绿植物 (Chlorophyta) 是一种植物.表达方式表达方式表达对于FeSOD来说,这是一个很好的方法.凝活动凝活动这种SOD的异构形式.

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