纳米颗粒促进巨核细胞的成熟和分化:对血液学平衡的潜在影响
Xiaoting Jin1,2, Ze Zhang2, Ge Guan2
1State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, P. R. China.
ACS applied materials & interfaces
|June 13, 2023
概括
纳米颗粒 (SiO2NP) 促进巨核细胞的发育和血小板的形成,而较小的尺寸会产生更强的效应. 这项研究强调了与影响血液平衡的SiO2NP相关的潜在健康风险.
科学领域:
- 纳米毒理学研究
- 血液学 血液学 血液学
- 生物医学工程 生物医学工程
背景情况:
- 纳米颗粒 (SiO2NP) 被广泛使用,导致环境释放和潜在的健康问题.
- 血液平衡的障碍,特别是血小板水平,与心血管疾病有关.
- 了解纳米材料的血液兼容性至关重要,血小板形成调节是一个关键方面.
研究的目的:
- 研究不同大小的SiO2NP对巨核细胞成熟和分化成血小板的影响.
- 探索SiO2NP对血液平衡的尺寸依赖性毒理影响.
- 阐明SiO2 NP诱导的血小板产生的变化背后的分子机制.
主要方法:
- 巨核细胞暴露于不同大小 (80, 120, 200, 400 nm) 的SiO2NP.
- 评估巨核细胞形态,DNA含量,DNA化和CD41a的表达.
- 分析了参与巨核形成的关键基因 (GATA-1,FLI-1,aNF-E2,fNF-E2) 的转录表达.
- SiO2 NP大小与观察到的生物效应之间的相关性分析.
主要成果:
- SiO2 NPs促进了巨核细胞的发展,由细胞形态变化,大小增加,DNA含量增加和性增长所证明.
- 在SiO2 NP治疗后观察到巨核细胞特异性抗原CD41a的升调.
- 较小的SiO2NP在巨核细胞的成熟和分化上表现出更强的作用.
- GATA-1和FLI-1表达的上调与巨核细胞成熟正相关,而aNF-E2和fNF-E2保持不变.
结论:
- SiO2 NPs可以通过促进巨核细胞的成熟和分化来扰乱血小板参与的血液静止.
- SiO2NP的大小是影响其血液毒理作用的关键因素,较小的颗粒更强效.
- GATA-1和FLI-1在观察到的SiO2NP诱导的巨核形成中起着重要作用,表明潜在的健康风险.
更多相关视频
09:46Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells
Published on: December 27, 2017
19.8K
08:13Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles
Published on: February 27, 2021
4.6K
相关概念视频
Role of Hematopoietic Growth Factors
1.4K
Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
Thrombopoietin (TPO), mainly released by the liver,...
1.4K
Regulation of Hematopoietic Stem Cells
3.2K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.2K
Hematopoiesis
5.4K
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
5.4K
Multipotency of Hematopoietic Stem Cells
3.2K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.2K
