相关实验视频
Updated: Jul 26, 2025

08:15
Metabolomic Analysis of Barley by Gas Chromatography/Mass Spectrometry
Published on: November 8, 2024
487
在DMS分离过程中可能发生化学转变:从酒的苦味化合物中吸取教训
Christian Ieritano1,2,3, Alexander Haack1,2, W Scott Hopkins1,2,3,4
1Department of Chemistry, University of Waterloo, 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada.
Journal of the American Society for Mass Spectrometry
|June 13, 2023
概括
异醇 (IPA) 添加到气流中,意外地导致隆 tautomers 在分离过程中合并. 离子移动装置内发生了微溶和分体化,影响了分离分辨率.
科学领域:
- 分析化学 分析化学
- 物理化学 物理化学
- 分离科学 分离科学
背景情况:
- 开发酒中苦化合物的分离方法,特别是使用漂流管质谱法 (DMS) 的humulone tautomers.
- 在与异醇 (IPA) 的环境中观察到素化隆分离体 ([Hum + Ag]+) 的部分分离.
研究的目的:
- 调查在DMS中引入溶解气体时,对humulone tautomers的分辨率损失.
- 了解IPA在离子过渡过程中的动态聚类,微溶和分体化中的作用.
主要方法:
- 碰撞引起的解离 (CID)
- 紫外线光解离谱学 紫外线光解离谱学
- -交换 (HDX) 是一种
- 离子移动光谱学 (IMS) 模拟
主要成果:
- 使用CID,UVPD和HDX确认了二enol,cis-keto和trans-keto tautomers的分配.
- HDX表示由IPA-humulone离子集群刺激的质子转移.
- IPA积累导致稳定的微离子,影响补偿电压 (CV).
- 微溶解介导的丁醇在DMS过境过程中转基因分体化.
结论:
- 添加IPA和随后的微溶解和分体化显著影响了离子移动性分离.
- 由于微溶解,CV响应的差异导致 cis-和 trans-keto humulone tautomers 的峰值凝聚.
- 在离子移动装置中观察到/醇分离,这是一个新发现.
相关概念视频
Mass Spectrometry: Alcohol Fragmentation
3.6K
Alcohols (R-OH) ionize to lose one non-bonded electron from the oxygen atom, forming molecular ions. Due to their tendency to fragment rapidly, the intensity of the molecular ion peak in the mass spectrum is weak or sometimes absent. The fragmentation patterns for alcohols occur in two ways, i.e. ⍺-cleavage and dehydration. During ⍺-cleavage, the bond at the ⍺-position adjacent to the hydroxyl group cleaves to give a resonance-stabilized cation and a radical. However,...
3.6K
Optimizing Chromatographic Separations
441
Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
441
Extraction: Advanced Methods
497
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
497
Detergent Purification of Membrane Proteins
5.2K
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
5.2K
Distillation: Vapor–Liquid Equilibria
2.9K
Distillation is a separation technique that takes advantage of the boiling point properties of disparate elements in a mixture. To perform distillation, we begin by heating a miscible mixture of two liquids with a significant difference in boiling points (at least 20°C). As the solution heats up and reaches the bubble point of the more volatile component, some molecules of the more volatile component transition into the gas phase and travel upward into the condenser, which is a glass tube...
2.9K
Dialysis
737
Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...
737

