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Qiao-Ran Sun^1,2, Dan-Ya Wu^1,2, Jing-Jing Zhang^1,2

¹Institute of Stem Cell and Regenerative Biology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.

Reproduction (Cambridge, England)

|June 13, 2023

概括

尼古丁暴露会损害早期胚胎发育,增加DNA损伤并减少胚胎细胞形成. 它还通过过度激活Notch信号通路导致胎盘异常.

科学领域:

发展生物学发展生物学
毒理学毒理学毒理学
生殖科学生殖科学

背景情况:

尼古丁是一种室内空气污染物,在香烟烟雾中发现.
尼古丁的脂友性质允许快速在全身分布.
早期胚胎尼古丁暴露对发育的影响尚不清楚.

研究的目的:

研究尼古丁对早期胚胎发育和胎盘形成的影响.
阐明尼古丁诱导的发育异常背后的分子机制.
探索尼古丁诱导的胎盘问题潜在的治疗干预措施.

主要方法:

在暴露于尼古丁的胚胎中评估胚胎形成,活性氧物种,DNA损伤和亡.
分析尼古丁暴露后的胎盘体重和结构.
调查Phlda2促进体甲基化和mRNA表达.
利用RNA测序来识别受影响的基因表达和信号通路.
使用DAPT来阻止Notch的信号通路.

主要成果:

尼古丁暴露减少了囊胚细胞的形成,增加了氧化应激,DNA损伤和亡.
尼古丁暴露导致胎盘体重增加和结构性破坏.
观察到Phlda2促进物的高甲基化和Phlda2mRNA表达的减少.
尼古丁暴露过度激活了诺奇信号通路.
使用DAPT阻断Notch信号扭转了尼古丁诱导的胎盘异常.

结论:

尼古丁暴露对早期胚胎质量和胎盘发育产生负面影响.
过度激活Notch信号通路是尼古丁诱导的胎盘异常的一个关键机制.
针对Notch信号通路可能提供一种策略,以减轻尼古丁对怀孕的不良影响.

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