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工程Saccharomyces cerevisiae YPH499 对于过度生产的日拉尼尔日拉尼醇

Junhua Wang^1,2,3,4, Youran Li^1,2,3, Wei Jiang^1,2,3,5

¹Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, People's Republic of China.

Journal of agricultural and food chemistry

|June 13, 2023

概括

研究人员通过优化美酸盐路径和发酵,提高了酵母中的基拉尼尔基拉尼 (GGOH) 产量. 这项工作促进了GGOH的生产,用于二聚和四聚合成中的应用.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
合成生物学合成生物学

背景情况:

格拉尼尔格拉尼奥尔 (GGOH) 是二甲和四甲化合物的关键前体.
在微生物宿主 (如Saccharomyces cerevisiae) 中高效生产GGOH对于工业应用至关重要.
为最大限度地提高GGOH产量,优化基拉尼尔基拉尼尔二酸盐 (GGPP) 的供应和转化效率至关重要.

研究的目的:

为了改造*Saccharomyces cerevisiae*用于增强的基拉尼尔基拉尼 (GGOH) 生产.
为了改善格拉尼尔格拉尼尔二酸盐 (GGPP) 的代谢途径,提高GGOH标位.
为大规模GGOH生产优化发酵策略.

主要方法:

在美酸盐 (MVA) 途径中过度表达基因以促进素生产.
*PaGGPPs*-*ERG20*和*PaGGPPs*-*DPP1*的同时表达,并对*ERG9*进行下调调节,以重定位代谢流.
引入依赖NADH的HMG-CoA还原酶 (*SpHMGR*),以平衡辅因子依赖.
在5L生物反应器中优化料批发发酵.

主要成果:

工程酵母在摇瓶水平上产生了597.12 mg/L的GGOH.
在路径修改后,GGOH标位增加到1221.96 mg/L.
通过引入*SpHMGR*,进一步提高到1271.14 mg/L.
在5L生物反应器中达到6.33g/L的最终GGOH标位,改善了24.9%.

结论:

代谢工程策略显著提高了Saccharomyces cerevisiae中的GGOH产量.
优化发酵过程对于达到高位的GGOH至关重要.
这项研究为开发酵母细胞工厂的发展提供了一个强大的平台,用于二甲和四甲生产.

关键词:

这种植物是Saccharomyces cerevisiae.杰拉尼尔杰拉尼奥尔 (geraniol) 是一种杰拉尼尔杰拉尼尔二酸盐合成酶梅瓦诺酸的途径斯卡瓦林酸盐是一种酸盐 (squalene).

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