在Lactuca中Verticillium dahliaeLactuca spp中VdLs17VdLs17VdLs17Verticillium
Santosh Nayak1, Kelley Richardson1
1Crop Improvement and Protection Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Salinas, CA 93905.
Plant disease
|June 13, 2023
概括
菜对Verticillium枯的耐药性是由两个主要基因控制的. 由于复杂的遗传和环境因素,为了获得持久的耐药性,繁殖需要大量的种群和后代选择.
科学领域:
- 植物病理学 植物病理学
- 遗传学 是一个遗传学.
- 园艺科学 园艺科学
背景情况:
- 菜 (Lactuca sativa L.) 生产面临着由土壤传播的真菌Verticillium dahliae引起的Verticillium枯的重大威胁.
- 虽然对V. dahliae的第1种类存在耐药性,但过度依赖耐药品种可能会导致耐药性突破的分离物,从而损害植物的耐久性.
- 了解部分耐药性的遗传基础对于开发针对这种病原体的可持续育种策略至关重要.
研究的目的:
- 为了确定部分抵抗Verticillium dahliae的遗传模式,我们在Lactuca spp.中分离了VDLs17.
- 为了研究控制部分耐药性的遗传模型,使用F2:3后代从两个部分耐药的加入之间的交叉.
- 为设计有效的菜育种计划提供洞察力,以抵抗Verticillium枯.
主要方法:
- 从Lactuca serriola (11G99) 和Lactuca sativa (PI 171674) 的交叉中产生了258个F2:3后代,两者都部分耐药.
- 在受控的温室和生长室条件下,在3年内进行了8次实验,使用随机的完整块设计.
- 进行分离分析以阐明对V. dahliae部分耐药性的遗传控制,分离VdLs17.
主要成果:
- 对V. dahliae孤立VdLs17的部分耐药性是由两个主要基因遗传模型控制的.
- 遗传涉及附加性,主导性和表观性基因效应,表明复杂的遗传相互作用.
- 观察到过分分离,这表明父母系中存在有利和不利的等位基因.
结论:
- 开发具有耐久性抵抗Verticillium枯的生菜是具有挑战性的,因为表皮性基因效应和环境对疾病严重性的重大影响.
- 为了最大限度地捕获有利的添加基因,需要产生和评估大量种群,选择最好是在后代进行的.
- 这项关于部分耐药性遗传的研究结果对于为未来的生菜繁殖策略提供信息至关重要,以防止Verticillium wilt.
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