菌体结合的Aspergillus niger AKU 3302的原酸酸酶作为一个稳定的固定生物催化剂
Ana Paula Butiuk1, María Alicia Martos1, Roque Alberto Hours2
1School of Exact, Chemical and Life Sciences, Misiones National University, Félix de Azara 1552, N3300LQH Posadas, Misiones, Argentina.
Journal of bioscience and bioengineering
|June 13, 2023
概括
这项研究开发了一种稳定的Aspergillus niger生物催化剂,用于酸 (CGA) 水解,有效地从yerba mate中产生有价值的酸 (QA) 和咖啡酸 (CA). 生物催化剂显示出出色的可重复使用性和存储稳定性,适用于工业应用.
科学领域:
- 生物催化剂是一种生物催化剂.
- 酶技术 酶技术是一种
- 工业生物技术 工业生物技术
背景情况:
- 酸 (CGA) 的水解产生高价值化合物:酸 (QA) 和咖啡酸 (CA).
- 开发高效的生物催化剂用于CGA转化对于工业应用至关重要.
研究的目的:
- 为CGA水解制备和表征一种基于黑色菌的非活性的Aspergillus nigerCHase生物催化剂.
- 评估生物催化剂的稳定性,可重复使用性和有效性,用于从yerba mate残留物转化CGA.
主要方法:
- 准备非活性的Aspergillus niger AKU 3302菌株与细胞相关的CHase.
- 体的热处理以禁用生长,同时保持酶活性.
- 在不同的条件下 (温度,pH值,催化剂负载,质量转移) 评估CHase活性.
- 通过批量周期和存储稳定性评估运行稳定性.
主要成果:
- 该CHase生物催化剂在pH 6.5和50°C时表现出最佳活性,具有很高的热稳定性.
- 在55°C进行30分钟的热处理后,没有观察到CHase活动的丧失,这消除了菌根的生长.
- 生物催化剂表现出卓越的运行稳定性 (11个批次循环),并在储存25天后保持85%的活性.
- 通过使用带有生物催化剂的包装式反应器实现了CGA到QA和CA的有效生物转化.
结论:
- 一种新的,稳定的,可重复使用的CHase生物催化剂是从不可活的Aspergillus niger菌根中开发出来的.
- 这种生物催化剂提供了一种具有成本效益和高效的生物技术工艺,用于从yerba mate中生产QA和CA.
- 该过程表现出显著的操作和存储稳定性,使其适合工业规模实施.
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