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聚3-基酸盐) 通过Bacillus infantis sp. 的降解. 从土壤中分离出来,并识别出表达PHB脱聚合酶的phaZ和bdhA

Yubin Jeon¹, HyeJi Jin¹, Youjung Kong¹

¹Department of Biological and Chemical Engineering, Hongik University, Sejong 30016, Republic of Korea.

Journal of microbiology and biotechnology

|June 13, 2023

概括

从土壤中分离出来的三种新的Bacillus infantis菌株能够有效地降解聚基酸 (PHB) 生物塑料. 一个菌株B. infantis PD3在营养不良的条件下在5天内实现了98.71%的降解.

科学领域:

微生物学微生物学
生物技术是生物技术.
环境科学环境科学

背景情况:

聚3-基酸盐 (PHB) 是一种具有重大工业潜力的可生物降解和生物相容的生物塑料.
有效降解PHB,特别是在营养缺乏的环境中,对于其实际应用和可持续处置至关重要.
目前的PHB降解方法需要针对工业和堆肥应用进行优化.

研究的目的:

在营养缺乏条件下分离和识别能够降解PHB的新型微生物.
在分离的菌株中对PHB降解基因 (phaZ和bdhA) 的特征.
评估PHB被孤立的Bacillus infantis菌株降解的效率和物理影响.

主要方法:

使用PHB双层板隔离PHB降解细菌.
通过聚合酶链反应 (PCR) 识别细菌分离物和确认特定基因 (phaZ,bdhA).
在矿物质介质中使用PHB薄膜测定来量化PHB降解速率,并通过凝透色谱 (GPC) 和扫描电子显微镜 (SEM) 分析物理变化.

主要成果:

从土壤样本中成功分离出了三种具有PHB降解能力的新型Bacillus infantis菌株.
在所有孤立的B. infantis菌株中证实了phaZ和bdhA基因的存在.
在营养缺乏的矿物质介质中,Bacillus infantis PD3在5天内显示出98.71%的高PHB降解率,显著降低了分子重量和表面侵蚀.

结论:

婴儿杆菌具有显著的PHB生物降解潜力,特别是在具有挑战性的营养有限的条件下.
这项研究首次证明了Bacillus infantis中卓越的PHB降解能力,确定PD3菌株是高度有效的降解剂.
这些发现有望推动PHB的商业化,并有助于制定有效的工业堆肥策略.

关键词:

婴儿杆菌 (Bacillus infantis) 是一个在PHB脱聚合酶中,PHB脱聚合酶聚基酸) 是一种聚基酸.这就是为什么bdhAdhA 生物降解生物降解 phaZ phaZ 的意思

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