高质量的染色体水平de novo组装的三叶草复原
Hongjie Wang1,2, Yongqiang Wu1,2, Yong He1,2
1College of Grassland Science, Qingdao Agricultural University, Qingdao, 266109, China.
BMC genomics
|June 13, 2023
概括
使用PacBio Hi-Fi测序生成了一种高质量的白叶草基因组组. 这为了解这种重要的料豆类的分子育种和演变提供了至关重要的资源.
科学领域:
- 植物基因组学 植物基因组学
- 料作物科学 料作物科学
- 豆类生物学 豆类生物学
背景情况:
- 白三叶草 (Trifolium repens L.) 是一种有价值的多年性豆类料,具有重要的营养,生态和药用意义.
- 它的全四形性质和可取的农学特征,包括对环境压力的耐受性,使其被广泛种植.
- 缺乏参考基因组阻碍了白叶三叶草的繁殖和栽培方面的进步.
研究的目的:
- 为了生成高质量的,染色体水平的白三叶草新基因组组合.
- 为了注释白三叶草基因组并识别基因模型.
- 为分子育种和白三叶草的进化研究提供基础资源.
主要方法:
- 利用PacBio第三代Hi-Fi测序来进行基因组装.
- 执行了白三叶草基因组的新组合和注释.
- 进行了遗传学分析和基因家族扩张/收缩分析.
主要成果:
- 产生了1096 Mb的白色三叶草基因组组合,具有高连续性 (N50 = 14 Mb) 和完整性 (BUSCO = 98.5%).
- 标注了90,128个高可靠性基因模型,揭示了与环境抵抗和农业特征相关的基因家族扩展.
- 与Trifolium pratense和Trifolium介质建立了密切的进化关系.
结论:
- 通过使用PacBio Hi-Fi测序,成功生成了白叶草基因组的高质量,染色体水平的de novo组件.
- 这种基因组组装是加速白三叶草研究和分子育种的关键资源.
- 这些基因组数据将有助于未来对豆类料生物学的研究,进化和定量特征局部映射.
相关概念视频
03:36Genome Annotation and Assembly
18.9K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.9K
02:27Trihybrid Crosses
23.5K
Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...
23.5K