基于RNA的amplicon测序在测量环境微生物群落中的代谢活动方面是无效的
Ya Wang1,2,3, Kelsey N Thompson1,2,3, Yan Yan1,2,3
1Department of Biostatistics, Harvard T.H. Chan School of Public Health, Harvard University, 665 Huntington Avenue, Boston, MA, 02115, USA.
Microbiome
|June 13, 2023
概括
基于16S rRNA转录的amplicon测序 (16S-RNA-seq) 可以评估简单的社区中的微生物活动,但与复杂的社区作斗争. 这种方法在现实的环境样本中提供了取决于分类群的相对可行性见解.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 描述微生物活动对于理解微生物组功能至关重要,但目前基于DNA的方法无法区分活细胞和死细胞.
- 基于16S rRNA转录的amplicon测序 (16S-RNA-seq) 是评估活跃微生物群落的拟议方法,但其有效性需要系统评估.
- 这项研究对基于RNA的amplicon测序进行了基准,用于在合成和环境样本中评估微生物活性.
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