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Updated: Jul 26, 2025

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

了解使用单分子技术的G-四重复生物和稳定性

Nicholas Kusi-Appauh¹, Stephen F Ralph¹, Antoine M van Oijen¹

¹Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia.

The journal of physical chemistry. B

|June 13, 2023

概括

单分子力技术揭示了G-四重复 (qDNA) 稳定性如何影响DNA维护. 这些方法可视化了像RPA和酶这样的蛋白质如何展开qDNA路障,这对基因组稳定至关重要.

科学领域:

生物化学和分子生物学
基因组学和表观遗传学
生物物理学的生物物理.

背景情况:

G-四重复 (qDNA) 结构与真核生物基因组维护有关.
它们的化学和机械稳定性影响与细胞机械的相互作用.
了解这些动态是理解DNA处理的关键.

研究的目的:

审查基于单分子力技术如何阐明qDNA的机械稳定性.
在压力下探索qDNA形状的相互转换.
研究qDNA稳定性在DNA维护和蛋白质相互作用中的作用.

主要方法:

原子力显微镜 (AFM) 的使用
磁性和光学 tweezers 的子.
单分子光共振能量转移 (smFRET) 是一种

主要成果:

基于力量的技术揭示了各种qDNA结构的机械稳定性.
结合体稳定的qDNA结构表现出改变的机械特性.
细胞蛋白 (RPA,BLM,Pif1) 可以展开qDNA结构.
smFRET阐明了qDNA解的蛋白质机制.

结论:

单分子工具可以直接可视化qDNA路障.
qDNA稳定性显著影响核机械绕过这些结构的能力.
G四重复体可以限制与端粒相关的蛋白质的访问,从而影响基因组过程.

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Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Published on: March 18, 2017

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