Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

9.3K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.3K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

16.4K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
16.4K
Transcription Factors02:16

Transcription Factors

76.2K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
76.2K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

13.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

951
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
951
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

3.1K
3.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Mtg16/Eto2 tumor suppressor maintains and establishes repression by distinct mechanisms.

Molecular cell·2026
Same author

Publisher Correction: Adaptor-mediated recruitment of three dyneins to dynactin enhances force generation.

Nature cell biology·2026
Same author

Adaptor-mediated recruitment of three dyneins to dynactin enhances force generation.

Nature cell biology·2026
Same author

Mtg16 NHR1 mutations cause defects in lymphopoiesis and the response to anemia.

Experimental hematology·2025
Same author

The Power of Three: Dynactin associates with three dyneins under load for greater force production.

bioRxiv : the preprint server for biology·2025
Same author

Pharmacological restriction of genomic binding sites redirects PU.1 pioneer transcription factor activity.

Nature genetics·2024

相关实验视频

Updated: Jul 26, 2025

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

2.2K

对增强器生物学和功能的新兴见解.

Mirjam Arnold1, Kristy R Stengel1,2,3

  • 1Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY, USA.

Transcription
|June 14, 2023
PubMed
概括

研究人员探索新技术,以了解增强剂,控制基因表达的DNA元素的功能. 进步揭示了对增强器转录,3D组织和转录因子在基因调节和疾病中的作用的见解.

关键词:
在这种情况下,染色染色素我们的 eRNA eRNA.增强剂是一种增强剂.基因调节 基因调节 基因调节转录因子的转录因子

更多相关视频

Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture 4C
10:02

Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture 4C

Published on: April 29, 2020

6.7K
Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
10:46

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Published on: June 2, 2018

9.4K

相关实验视频

Last Updated: Jul 26, 2025

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

2.2K
Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture 4C
10:02

Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture 4C

Published on: April 29, 2020

6.7K
Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
10:46

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Published on: June 2, 2018

9.4K

科学领域:

  • 分子生物学分子生物学
  • 遗传学 遗传学是一种遗传学.
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 细胞类型特定的基因表达依赖于DNA增强剂和转录因子 (TF).
  • 功能障碍增强剂或TFs与癌症等疾病有关.
  • 增强剂具有特定的染色体特征和TF结合的特征.

研究的目的:

  • 审查了解增强器功能的最新技术进步.
  • 通过增强剂突出显示基因控制的分子机制的新见解.
  • 专注于关键领域,包括增强器转录,3D组织和TF依赖.

主要方法:

  • 使用基于测序的测定以向染色体特征 (例如,DNase过敏,H3K27ac) 的增强剂元素的识别.
  • 全基因组功能测试用于研究基因表达中的增强剂作用.
  • 审查最近的技术进步,为增强机制提供新的见解.

主要成果:

  • 技术进步已经彻底改变了全基因组增强剂的识别.
  • 新的方法扩大了对增强剂如何在空间时间上协调基因表达的理解.
  • 获得了对增强剂转录,增强剂-促进剂相互作用,3D基因组组织和TF/辅因子依赖性的见解.

结论:

  • 最近的技术进步显著提高了我们研究增强器功能的能力.
  • 了解增强器机制对于破译基因调节和疾病发展至关重要.
  • 未来的研究方向包括全基因组功能增强器屏幕和更深入地分析调节元素相互作用.