双倍平分体面包小麦种群的表型和遗传多样性以及对尖端特征,最终用途质量和生物强化能力的分子验证
Imren Kutlu1, Sadettin Çelik2, Yaşar Karaduman3
1Department of Field Crops, Faculty of Agriculture, Osmangazi University, Eskişehir, Turkey.
PeerJ
|June 14, 2023
概括
识别DNA标记物加速了高产量,高质量和营养丰富的面包小麦的育种. 这项研究验证了简单序列重复 (SSR) 标记器,以有效地选择理想的小麦基因型.
科学领域:
- 农业科学 农业科学
- 植物育种 植物育种
- 遗传学 是一个遗传学.
背景情况:
- 提高面包小麦的质量,营养价值和产量是一个关键的育种目标.
- 传统的育种方法耗时且受到环境因素的影响.
- DNA标记器提供了一种经济有效和快速的方法来识别优异的基因型.
研究的目的:
- 为了验证十个基因简单序列重复 (SSR) 标记器用于分子表征.
- 识别具有增强产量组件,质量特征和谷物铁 (Fe) 和 (Zn) 度的基因型.
- 为了评估一双平分体 (DH) 小麦种群中的遗传多样性和种群结构.
主要方法:
- 在两个赛季中,对134个DH线和4个父系进行了产量,质量和谷物Fe/Zn的表型评估.
- 使用十个经过验证的SSR标记物的基因型鉴定.
- 使用算术平均值 (UPGMA) 和结构的未加权对组方法对遗传多样性的分析.
主要成果:
- 对所有研究的特征观察到显著的基因型变异.
- 十个SSR标志物显示出显著的多态性,其中六个显示出高遗传多样性.
- 标记特征关联分析确定了与产量,质量和谷物矿物质含量相关的特定SSR.
- 在UPGMA和STRUCTURE分析中,138种基因型被分为五个不同的群体.
结论:
- 已验证的SSR标记物 (Xbarc61,Xbarc146,Xgwm282,Gwm445) 在标记物辅助选择 (MAS) 中是有效的.
- 这些标记可以加速面包小麦的发展,提高产量,质量和生物强化.
- 这项研究表明,分子标记在高效的小麦育种计划中具有实用性.
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