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使用主导突变基因Ae1-5180 (氨糖扩展剂) 开发高氨糖玉米

Jienan Han¹, Zenghui Guo^1,2, Meijuan Wang¹

¹Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Haidian District, Beijing, 100081 China.

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|June 14, 2023

概括

在玉米中占主导地位的Ae1-5180突变显著增加了粉含量 (AC) 并改变了粉的特性. 这项研究开发了用于高粉糖玉米育种的标记辅助选择,加速了这一过程.

科学领域:

植物遗传学和育种.
粉合成的生物化学

背景情况:

玉米粉是一种有价值的粉,具有多种应用.
粉分枝酶IIb (SBEIIb) 的突变是增加粉含量 (AC) 的关键.
主导的Ae1-5180等位基因在粉合成和繁殖潜力的作用需要进一步研究.

研究的目的:

描述Ae1-5180突变对玉米核和粉特性的影响.
调查Ae1-5180对粉合成酶活动的影响.
开发一种用于辅助选择的标记物,并评估Ae1-5180近同位素线 (NILs) 的繁殖潜力.

主要方法:

对Ae1-5180突变种子和粉颗粒的表型分析.
测量粉的热稳定性和氨酸聚合物的聚合.
在核发育过程中对粉合成的酶活性测定.
基于Mu1转位子插入的分子标记物 (mu406) 的开发.
对AC和100核重量的Ae1-5180 NILs的评估.

主要成果:

Ae1-5180突变核呈现高AC (47.23%),变色/玻璃般的外观,较小,不规则的粉颗粒,以及更高的热稳定性.
与野生类型相比,粉合成酶活动在Ae1-5180突变体中显示出动态变化.
一个功能性标记物 (mu406) 被开发用于选择Ae1-5180 NILs.
几只Ae1-5180 NIL显示出高AC (>40%) 和繁殖应用的潜力.

结论:

主导的Ae1-5180突变赋予了经典的高粉素特征,并改变了粉结构和合成.
开发的标记物有助于高粉糖玉米系的有效选择.
Ae1-5180作为一种有价值的遗传资源,可以加速高粉糖玉米的育种计划.

关键词:

Ae1-5180 突变变异的人氨糖糖是什么氨糖糖是什么玉米玉米玉米是一种标记器辅助的选择这样做是可以的.

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