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在PI 594756中在Rpp1位点绘制大豆抗性图

Luciane G Barros¹, Bruna B Avelino¹, Danielle C G da Silva²

¹Department of General Biology, State University of Londrina (UEL), Londrina, Paraná Brazil.

Molecular breeding : new strategies in plant improvement

|June 14, 2023

概括

研究人员在PI 594756.6中发现了一种新的大豆抗基因. 这种位于第18染色体上的基因为抗击巴西亚洲大豆生的标记器辅助选择提供了一个新的工具.

科学领域:

植物病理学植物病理学
遗传学是一个遗传学.
农业学是一种农业学.

背景情况:

由*Phakopsora pachyrhizi*引起的亚洲大豆生 (ASR) 是巴西大豆的主要疾病.
开发耐药大豆品种对于疾病管理和作物产量保护至关重要.

研究的目的:

调查和绘制大豆PI 594756.6中对*Phakopsora pachyrhizi*耐药性的遗传基础.
在育种计划中识别用于标记器辅助选择 (MAS) 的分子标记物.

主要方法:

使用大量分离分析 (BSA) 使用*F*₂和*F*_2:3种群,该种群来自抗性PI 594756和易受性PI 594891之间的交叉.
用Infinium BeadChips和目标GBS (tGBS) 进行基因型鉴定.
在全基因组测序-SNP数据库上进行了QTL映射和单种类型分析.

主要成果:

PI 594756表现出一种独特的抗ASR抗性特征,由单基因主导基因控制,从数量上看不完全主导.
耐药基因被映射到染色体18上的特定区域 (55,863,74156,123,516 bp),与已知的*Rpp*基因不同.
发现了新的SNP,区分PI 594756等位基因与*Rpp1*和*Rpp1-b*.

结论:

在PI 594756中发现并绘制了一种新的大豆抗性位.
已识别的SNP为标记器辅助选择提供了有价值的工具,以开发抗ASR的大豆品种.
这一发现有助于增强巴西大豆对"Phakopsora pachyrhizi"的抵抗力.

关键词:

遗传映射遗传映射是一种基因映射.甘氨酸最大值 (Glycine max) 是一种哈普洛型是指一种类型.帕科普索拉 (Phakopsora pachyrhizi) 是一种植物.Rpp Rpp 这是一个很好的方法.

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