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在面包小麦中对黑点耐药性的定量特征位点是由Bipolaris sorokiniana引起的

Chuang Gao¹, Gaili Song¹, Kefei Qu¹

¹National Engineering Research Centre for Wheat/College of Agronomy/Henan Technology Innovation Centre of Wheat/National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, 15 Longzihu College District, Zhengzhou, 450046 Henan Province China.

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|June 14, 2023

概括

研究人员为小麦黑点耐药性确定了五个定量特征位置 (QTL),包括5D染色体上潜在的新位置. 与这些QTL相关的分子标记物在耐繁殖的小麦品种中为标记器辅助选择 (MAS) 提供了希望.

科学领域:

植物病理学植物病理学
植物遗传学植物遗传学
农业科学农业科学

背景情况:

黑点病是由*Bipolaris sorokiniana*引起的,对全球小麦产量产生重大影响.
开发耐受性小麦品种对于减轻作物损失和确保粮食安全至关重要.

研究的目的:

确定主要的定量特征位点 (QTL),使小麦对黑点病产生抵抗力.
开发用于标记器辅助选择 (MAS) 的分子标记物,以加速黑点耐药性的繁殖.

主要方法:

在人工注射下,对复合同血统 (RIL) 种群的黑点耐药性进行了评估.
使用660K SNP数组进行大规模分离分析,确定了单核酸多态 (SNP).
使用SSR和dCAPS标记物来定位QTLs,构建了一个遗传链接地图.

主要成果:

在5A,5B和5D染色体上确定了黑点抗性五个QTL.
已识别的QTLs,被指定为*QBB.hau-5A*, *QBB.hau-5B*.1, *QBB.hau-5B*.2, *QBB.hau-5D*.1,和 *QBB.hau-5D*.2,所有这些都来自于Yuyou1父体的阻力.
*QBB.hau-5D*.1 是一个新的黑点阻力位置.
发现标记物*Xwmc654*和*Xgwm174*与5A和5D染色体上的抗性QTL有关.

结论:

已识别的QTL和相关标记物为增强黑点抵抗力的育种小麦提供了宝贵的遗传资源.
与新型位点*QBB.hau-5D*.1相关的标记符号*Xgwm174*显示了在MAS程序中应用的巨大潜力.
这项研究有助于开发可持续的策略,通过基因改进来管理小麦的黑点病.

关键词:

双极星索罗基尼亚纳纳纳黑点疾病是黑点疾病.标记器辅助的选择定量特征的位置 (locus) 是一个定量特征的位置.在Triticum aestivum中,它是最重要的.

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