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LRRC8A促进了结肠癌细胞中氧沙素耐药性的初始发展

Haifeng Zhang^1,2, Zhenghui Jing^1,2, Rong Liu^1,2

¹Department of Pathology of Basic Medicine College, Xi'an Jiaotong University, Xi'an 710061, China.

|June 14, 2023

概括

富含白的重复含有8A (LRRC8A) 通过增加其表达,促进结肠癌细胞对氧沙的抵抗. 然而,LRRC8A对于维持这种耐药性并非必不可少,这表明它在获得耐药性方面发挥了作用.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

富含白的重复含有8A (LRRC8A) 是体积调节离子通道 (VRAC) 的关键组成部分.
VRACs涉及至关重要的细胞过程,包括增殖,迁移,亡和耐药性.
了解LRRC8A在癌症抗药性中的作用对于开发有效疗法至关重要.

研究的目的:

研究LRRC8A在结肠癌细胞中抗氧化抗性的发展中的作用.
为了确定LRRC8A是否参与氧化抗性的获得或维持.

主要方法:

细胞计数套件-8 (CCK8) 测试细胞活力.
亡测定. 亡测定. 死亡测定.
RNA测序用于分析敏感细胞和耐药细胞之间的基因表达差异.
操纵LRRC8A表达以评估其对药物耐药性的影响.

主要成果:

与原生HCT116细胞相比,抗氧化结肠癌细胞 (R-Oxa) 呈现出显著增加的耐药性.
LRRC8A mRNA和蛋白质表达在抗氧化和缺乏药物的耐药细胞中显著增加.
调节LRRC8A表达影响了敏感细胞的氧沙抗性,但不影响耐药细胞的抗性.
的药物耐药性途径的转录调节可能有助于耐药性维护.

结论:

在结肠癌中,LRRC8A促进了对氧沙的耐药性.
LRRC8A对于维持已建立的氧化抗药性来说并不必不可少.
对的抗药性途径进行进一步的研究是有必要的.

关键词:

大肠癌是什么意思大肠癌是什么意思药物耐药性药物耐药性药物耐药性美国LRRC8AA 氧化酸是一种氧化酸.在VRAC中,VRAC就是VRAC.

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