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通过目标测序 (GBTS) 进行基因型鉴定的SNP标记面板的开发及其在大豆中的应用

Qing Yang¹, Jianan Zhang², Xiaolei Shi¹

¹Hebei Laboratory of Crop Genetics and Breeding, National Soybean Improvement Center Shijiazhuang Sub-Center, Huang-Huai-Hai Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture and Rural Affairs, Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, High-Tech Industrial Development Zone, 162 Hengshan St, Shijiazhuang, 050035 Hebei People's Republic of China.

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概括

通过目标测序 (GBTS) 方法的新型基因定型为大豆遗传研究提供了一个低成本,准确和灵活的平台. 这项技术有助于标记器辅助的选择,遗传映射和定量特征位点识别,加速作物改进.

科学领域:

植物遗传学植物遗传学
基因组学就是基因组学.
农业科学农业科学

背景情况:

高通量基因型鉴定对于大豆标记器辅助选择和遗传映射至关重要.
现有的方法需要优化,以提高成本效益和准确性.

研究的目的:

开发和验证一个灵活,准确和低成本的基因型定制通过目标测序 (GBTS) 平台为大豆.
评估GBTS不同SNP面板尺寸的性能.

主要方法:

从 GBTS 的 SoySNP 阵列中选择了三个测试面板 (41,541,20,748 和 9670 SNP 标记).
使用15个代表性加入和重新排序数据评估准确性和一致性.
用10K面板对100种种子重量进行基因型化父衍生种群和分析QTL.

主要成果:

GBTS的准确性很高,技术复制品与99.87%的技术复制品相同,40K面板与再测序之间98.86%.
基因型数据准确地反映了加入血统,并构建了链接地图.
确定了100种种子重量的稳定QTL (Locus_OSW_06) 并量化了与GBS和DNA芯片相比的成本降低.

结论:

低成本的GBTS面板对于大豆生殖质的评估,链接地图的构建和QTL识别是有效的.
这个平台可以促进基因组选择,加速大豆育种计划.
开发的GBTS方法为推进大豆遗传学研究提供了有价值的工具.

关键词:

通过目标测序 (GBTS) 进行基因型鉴定.胚胎细胞质评价的评价用标记器辅助的选择 (MAS)在 QTL 识别中使用 QTL 识别.在SNP标记面板上,SNP标记面板.在大豆繁殖中培育大豆.

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相关概念视频

Single Nucleotide Polymorphisms-SNPs

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...