在低通测序数据中增加的异构性允许识别牛群中的血嵌合体
Dorothea Lindtke1, Franz R Seefried2, Cord Drögemüller3
1Agroscope, Posieux, Switzerland.
Animal genetics
|June 14, 2023
概括
低通序测可靠地检测牛血液中的遗传奇梦,比传统方法识别更多病例. 这种方法有助于理解生殖挑战,并突出血液.
科学领域:
- 遗传学和基因组学 遗传学和基因组学
- 生殖生物学 生殖生物学
- 动物科学 动物科学
背景情况:
- 牛的多重怀孕通常会导致共享血液循环,从而导致遗传混沌.
- 异性恋双胞胎中的遗传奇梦可能会对生殖能力产生负面影响.
- 早期检测奇默症通常需要专门的,往往复杂的测试方法.
研究的目的:
- 评估低通序列测试在牛群中检测基因嵌合物的有效性.
- 为了将低通序列与SNP微阵列分析进行比较,以检测嵌合体.
- 评估金马主义的流行及其对牛的生殖结果的影响.
主要方法:
- 来自322个牛血液样本的低通序列数据 (0.64×中位覆盖率) 的分析.
- 基于基因组范围内的异构性增加的基因组基因组的基因组鉴定.
- 与来自同一动物的77个发泡样本的SNP微阵列数据进行比较.
主要成果:
- 低通测序确定了20个假定的血嵌合体,而SNP微阵列数据没有显示出嵌合体的证据.
- 在测序和微阵列数据之间观察到显著的基因型异常.
- 在18组双胞胎中,在15组中检测到了奇美主义,而5名所谓的单身双胞胎也表现出奇美主义,这表明共同双胞胎在子宫内的死亡率很高.
结论:
- 低通测序提供了一种可靠和有效的方法来选牛群中的血嵌合体.
- 血液不是一个合适的DNA来源,用于检测生殖系变异,由于奇默主义.
- 这些发现支持对牛子宫内同胞死亡率的更高估计.
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