全基因组高通量染色体构造捕获分析揭示了早期体质胚胎发生过程中的层次性染色体相互作用
Yan Chen1, Dejian Xie2, Xiangwei Ma1
1Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
Plant physiology
|June 14, 2023
概括
早期的体质胚胎发生涉及染色质重组,长端重复逆转移体在重组中发挥作用. 这个过程揭示了基因调节网络,这些网络对于长期细胞壁加厚至关重要.
科学领域:
- 植物生物学 植物生物学
- 基因组学就是基因组学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 身体胚胎发生 (SE) 是一个发展过程,反映了胚胎的胚胎发生.
- 早期的SE对于启动染色体重编程至关重要.
- 以前的研究表明染色体可访问性发生了变化,但缺乏3D结构数据.
研究的目的:
- 为了研究长 (Dimocarpus longan) 早期体质胚胎发生期间的3D染色质结构.
- 确定参与SE过程中细胞壁加厚的分子机制和调节网络.
- 为长期提供染色体水平的基因组组件.
主要方法:
- 用PacBio测序和Hi-C架构进行染色体级基因组组装.
- 对染色体构造,可访问性,H3K4me1修饰和转录的分析.
- 长端重复逆转移子 (LTR-RTs) 和转录因子 (TFs) 的识别.
主要成果:
- 一个446 Mb的染色体级基因组组件被在15个支架上被生成.
- 染色质动态显示了度和脱凝,在相互作用区域中LTR-RTs被丰富.
- 在早期的SE过程中观察到从A到B区的转化和增强的B-B相互作用.
- 确定了一种涉及H3K4me1修饰,乙烯反应因子TFs和细胞壁加厚的基因调控网络.
结论:
- 这项研究揭示了长期早期SE期间的3D染色质构造.
- 在这个过程中,LTR-RTs与染色质重组有关.
- 涉及TF和表观遗传修饰的监管网络推动了SE的细胞壁加厚.
- 这些发现提供了关于植物体质胚胎发生的分子机制的见解.
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