使用子载体疫苗对Helicobacter pylori感染进行预防性免疫
Paidamoyo M Katsande1, Van Duy Nguyen2, Thi Lan Phuong Nguyen3
1Department of Biological Sciences, Royal Holloway University of London, Egham, UK.
Helicobacter
|June 14, 2023
概括
工程 Bacillus 子表达 Helicobacter pylori 抗原提供了一个有希望的粘膜体疫苗策略. 这种方法显著减少了小鼠中的H. pylori殖民,解决了一个主要的公共卫生问题.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 疫苗开发 疫苗开发
背景情况:
- 杆菌感染是一个重要的全球健康问题,导致胃肠道疾病和增加胃癌风险.
- 对H. pylori的抗微生物治疗正在推动抗微生物耐药性 (AMR),需要使用替代策略.
- 目前没有针对H. pylori感染的疫苗.
研究的目的:
- 为了工程 Bacillus subtilis 子以显示 H. pylori 保护性抗原 (UreA 和 UreB).
- 在小鼠模型中评估这些工程子作为粘膜疫苗的免疫性和有效性.
主要方法:
- 细菌细菌子被基因改造,在它们的表面表达H. pylori尿酶亚单元A (UreA) 和B (UreB).
- 小鼠被口服免疫,用工程子进行免疫.
- 通过测量抗原特异性粘膜反应 (便sIgA),血清转换和超免疫性来评估免疫力.
- 在与细菌进行挑战后,H. pylori的殖民被量化.
主要成果:
- 使用表达UreA或UreB的子进行口服免疫,诱导了抗原特异性粘膜免疫反应.
- 在挑战后免疫小鼠中观察到H. pylori殖民的显著减少 (高达1-log).
- 工程子显示出诱导保护性免疫的潜力.
结论:
- 细菌子是开发针对H. pylori的粘膜疫苗的可行平台.
- 细菌子的热稳定性和益生菌的使用使它们成为H. pylori预防或治疗的有吸引力的候选人.
- 这种基于子的疫苗接种策略显示出控制H. pylori感染和打击AMR的前景.
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