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在人类中 Calpain 特定的分解片段

Kana Orihara¹, Momoko Kobayashi-Otsugu¹, Emi Nakajima^1,2

¹Senju Laboratory of Ocular Sciences, Senju Pharmaceutical Corporation Ltd., Portland, OR, USA.

Histology and histopathology

|June 14, 2023

概括

用SBDP150表示的calpain激活在人类中被发现,这表明它在与年龄相关的黄斑变性 (AMD) 和潜在的治疗点中的作用.

科学领域:

眼科医生眼科眼科
细胞生物学细胞生物学
生物化学生物化学

背景情况:

含有蛋白质分解片段的细胞外杂质是衰老和与年龄相关的黄斑变性 (AMD) 的标志.
局部缺氧是一种潜在的风险因素,有助于AMD的发病.
据假设,calpains是一种蛋白质分解酶,在缺氧后会导致视网膜细胞和视网膜色素表皮 (RPE) 退化,但在AMD中缺乏直接证据.

研究的目的:

为了从人类眼睛中识别drusen中的calpain分裂蛋白质.
调查与正常衰老和AMD相关的Drusen中calpain激活标志物的存在.

主要方法:

从正常和AMD捐赠眼睛分析了76个人类眼睛 (结节和软) 液体.
用于SBDP150的免疫光染色,这是calpain激活的特定标记物 (α谱的分裂产物).
免疫光染色用于recoverin以识别光受体细胞.

主要成果:

检测到SBDP150在80%的正常眼睛的节节结结结和90%的AMD眼睛.
SBDP150存在于72%的软德鲁森,主要来自AMD眼睛.
大多数来自AMD捐赠者的软体和结节性毒液都对SBDP150.0呈阳性染色.

结论:

卡尔帕因激活的标记物SBDP150首次在人类中被发现.
结果表明,calpain诱导的蛋白解有助于光受体和/或RPE细胞衰变在衰老和AMD.
卡尔帕因抑制剂是缓解AMD进展的潜在治疗策略.

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