感染传播的演变促进了mcr-1等离子体的持久性
Jun Yang1,2, Renjie Wu1, Qiang Xia3
1College of Veterinary Medicine National Risk Assessment Laboratory for Antimicrobial Resistant of Microorganisms in Animals, Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, Key Laboratory of Zoonosis of Ministry of Agricultural and Rural Affairs South China Agricultural University , Guangzhou, China.
mBio
|June 14, 2023
概括
由单个突变驱动的等离子体传播增加,改善了细菌中抗生素耐药性基因的持久性. 这表明,向结合可以对抗抗性的传播.
科学领域:
- 微生物学 微生物学
- 进化生物学 进化生物学
- 遗传学 是一个遗传学.
背景情况:
- 结合性等离子体是细菌进化和抗生素耐药性传播的关键驱动因素.
- 等离子体通常会给宿主细菌带来健康成本,影响它们的生长率.
- 补偿突变可以减轻这些健康成本,增强等离子体持久性.
研究的目的:
- 调查增强等离子体传输是否可以改善昂贵的抗生素耐药性等离子体的持久性.
- 通过实验进化一个不稳定的mcr-1等离子体并分析其进化适应.
- 评估结合率在维持等离子体传播抗生素耐药性的作用.
主要方法:
- 在实验室条件下mcr-1等离子体pHNSHP24的实验演变.
- 塑体群动态建模以评估塑体维护.
- 等离子体入侵实验,以测量入侵无等离子体群体的能力.
主要成果:
- 进化的等离子体pHNSHP24在36天后由于traJ基因的5'UTR.的突变 (A51G) 而表现出更好的持久性.
- 这种突变通过增强结合率显著增加了传染性等离子体的传播.
- 增加的结合补偿了等离子体的损失,对于维持mcr-1等离子体至关重要.
结论:
- 进化增强的传染性传播,不仅仅是补偿性突变降低了健身成本,可以改善抗生素耐药性等离子体的持久性.
- 抑制结合过程可能是一个可行的策略,以打击抗生素耐药性等离子体的传播.
- 一个单一的突变可以极大地改变等离子体的传染性和持久性,突出显示了快速的进化适应.
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