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低水平的抑制性组织蛋白标记在神经干细胞中微调基因转录

Arjun Rajan¹, Lucas Anhezini¹, Noemi Rives-Quinto¹

¹Life Sciences Institute, University of Michigan-Ann Arbor, Ann Arbor, United States.

|June 14, 2023

概括

无果C (FruC) 转录因子通过在监管区域中丰富H3K27me3来微调干细胞基因转录. 这种机制确保了正确的神经原始细胞的分化,并且可以在物种之间保存.

科学领域:

发展生物学发展生物学
干细胞生物学干细胞生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

干细胞的分化依赖于精确的基因调节.
转录和翻译控制协调基因活动.
由于转化补偿,微调干性基因转录的机制仍然不清楚.

研究的目的:

定义微调干细胞基因转录在神经干细胞中的机制.
调查无果C (FruC) 在神经原生细胞身份认同中的作用.

主要方法:

使用的神经干细胞 (神经母细胞) 和中介神经祖先 (INP) 认同承诺.
分析了FruC与cis调节元素的结合.
评估了FruC功能的改变和Polycomb Repressive Complex 2活性对基因表达和细胞分化的影响.

主要成果:

FruC 结合了神经细胞特异性基因的 cis 调节元件.
失去FruC功能本身不会影响INP的承诺.
减少的翻译控制与FruC功能的丧失相结合,导致INP脱差.
在cis调节区域,FruC通过低水平的H3K27me3丰富来负面调节基因表达.
减少多抑制复合体2活性通过增加干性基因活性来模仿FruC功能丧失.

结论:

低水平的H3K27me3丰富微调干细胞中的基因转录.
这种表观遗传机制对于维持茎状性和调节分化至关重要.
鉴定出来的机制是从到人类的潜在保护机制.

关键词:

D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. melanogaster. D. melanogaster. melanogaster. D. melanogaster. D. melanogaster. melanogaster. D.这种植物是Drosophila.没有口,没有口.不对称的划分不对称的划分.发育生物学是发展生物学.精细调整精细调整神经细胞质神经细胞质.多组复合镇压复合体 2 2 再生医学是一种再生医学.干细胞是干细胞的组成部分.

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