缺铁调节细菌体的代谢格局,促进其在炎症期间的弹性
Janina P Lewis1,2,3, Qin Gui1
1Philips Institute for Oral Health Research, Virginia Commonwealth University, Richmond, Virginia, USA.
Microbiology spectrum
|June 14, 2023
概括
细菌通过保守的机制适应低铁条件,铁作为主调节器,在宿主中持久. 这项研究定义了关键Bacteroidetes物种的铁刺激剂.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 主体与微生物的相互作用
背景情况:
- 细菌体是口腔和肠道微生物群中的关键无氧细菌.
- 铁对于细菌的生存至关重要,但细菌类的适应机制尚不清楚.
- 了解铁的调节是解释宿主体内的细菌持久性的关键.
研究的目的:
- 为了研究口腔和肠道中的铁刺激物种Bacteroidetes.
- 为了确定保存和独特的铁调节基因和途径.
- 为了将体外发现与宿主相关研究中的体内数据相关联.
主要方法:
- 在缺铁和补铁条件下对 Porphyromonas gingivalis,Prevotella intermedia 和 Bacteroides thetaiotaomicron 的转录组分析.
- 比较基因组学以确定保存和新的铁调节机制.
- 生物信息分析以将结果与现有的宿主相关的转录组数据进行比较.
主要成果:
- 鉴定了保存的铁调节基因,包括上调的黄素 (fldA) 和血红素吸收 (hmu),以及下调的铁素 (frd) 和酸脱酶 (sdh).
- 在B. thetaiotaomicron中发现了属性特定的机制,如碳水化合物代谢 (sus) 和异体利用 (xusABC).
- 仅在B. thetaiotaomicron中发现了酸盐减少酶 (nrfAH) 操作子的铁依赖调节,研究的基因与大肠炎研究的基因有显著的重叠.
结论:
- 铁是 Bacteroidetes 中基因表达和细菌持久性的主调节者.
- 许多铁平衡机制在口腔和肠道细菌群中得到保护.
- 这项工作为了解 Bacteroidetes 中的铁调节和宿主适应提供了基础.
关键词:
细菌菌类是这样的小微生物.细菌菌体 (Bacteroidetes) 是一种细菌体.葡萄牙牙牙病 Porphyromonas gingivalis 这种虫子是在此之前,Prevotella是中间的.主体的免疫反应是主体的免疫反应.铁的代谢 铁的代谢铁器规则 铁器规则 铁器规则铁的吸收 铁的吸收更多相关视频
11:04The Citrobacter rodentium Mouse Model: Studying Pathogen and Host Contributions to Infectious Colitis
Published on: February 19, 2013
26.5K
08:58Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis
Published on: January 5, 2017
12.3K
相关概念视频
Microbial Nutrition
115
Organisms exhibit remarkable metabolic diversity, categorized based on how they acquire energy and carbon. These strategies enable survival in various ecological niches and are essential for maintaining energy flow and nutrient cycling within ecosystems.Energy and Carbon SourcesOrganisms are classified as phototrophs or chemotrophs based on energy acquisition. Phototrophs use light as their energy source, while chemotrophs rely on oxidizing chemical compounds. Further differentiation arises...
115
Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy
362
Various diagnostic tests are employed in the diagnostic process for Inflammatory Bowel Disease (IBD), particularly to differentiate between Crohn's disease and ulcerative colitis.
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
362
Transduction
49
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...
49
Sulfur Assimilation
45
Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to...
45
Bacterial Phylum Bacteroidota
50
The phylum Bacteroidota includes over 700 species classified into four primary orders: Bacteroidales, Cytophagales, Flavobacteriales, and Sphingobacteriales. These gram-negative, non-sporulating rods exhibit saccharolytic capabilities and can be aerobic or fermentative, encompassing obligate aerobes, facultative aerobes, and obligate anaerobes. Many species display gliding motility, though some are nonmotile or use flagella. The genus Bacteroides is well-studied due to its significant role in...
50
Defense Against Bacterial Pathogens
1.5K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.5K
