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相关概念视频

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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基于高精度,低复杂性,高分辨率显微镜的细胞分类.

Tobias Gerling1,2, Neus Godino1, Felix Pfisterer1

  • 1Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses IZI-BB, Am Muehlenberg 13, 14476 Potsdam, Germany. michael.kirschbaum@izi-bb.fraunhofer.de.

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概括

这项研究引入了一种低复杂度的微流体系统,用于高分辨率的图像激活细胞分类,从而有效地隔离具有高纯度和活力的特定细胞类型.

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Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors
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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
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科学领域:

  • 生物医学工程 生物医学工程
  • 细胞生物学 细胞生物学
  • 微流体学 微流体学

背景情况:

  • 图像激活细胞分类 (IACS) 利用细胞特征来分离专门的细胞.
  • 目前的IACS方法面临图像质量和实验复杂性的局限性,阻碍了广泛采用.

研究的目的:

  • 开发一个简化,高性能的IACS系统.
  • 为了提高图像分辨率和处理时间,提高细胞分类准确度.
  • 为了证明该系统在基于亚细胞局部化的基础上隔离可活的T细胞的能力.

主要方法:

  • 一种新型的微流体装置,集成高数值孔径广场显微镜.
  • 精确的电离体细胞操纵用于受控的处理.
  • 先进的图像分析允许延长处理时间 (数百毫秒).

主要成果:

  • 在IACS中实现了前所未有的图像分辨率216nm.
  • 成功地根据>80%纯度的亚细胞光信号对活T细胞进行了分类.
  • 已证明高细胞恢复率 (85%) 和经过分类后确认的细胞活力.

结论:

  • 开发的微流体方法为IACS提供了低复杂度,高分辨率的解决方案.
  • 这个系统克服了以前的局限性,使IACS成为一个更容易访问和更强大的工具.
  • 该方法确保了分类细胞的高纯度,产量和活力,从而推进了细胞隔离技术.