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从Runella slithyformis获得的新型曼诺-2-表皮酶的基质特异性和活性的结构洞察

Hang Wang¹, Xiaomei Sun¹, Wataru Saburi²

¹Faculty of Advanced Life Science, Hokkaido University, Kita 10, Nishi 8, Kita-ku, Sapporo 060-0810, Japan.

Acta crystallographica. Section D, Structural biology

|June 14, 2023

概括

曼诺2-酶 (ME) 酶结构揭示了D-曼诺生产的独特循环机制. 这项研究阐明了酶的基质识别和单糖类表皮化催化过程.

科学领域:

生物化学生化学
酶学是一种酶学.
结构生物学结构生物学

背景情况:

曼诺-2-表皮酶 (ME) 是一种亚糖胺2-表皮酶 (AGE) 超级家族成员,具有产生D-曼诺的潜力.
对于ME的基质识别和催化机制尚不清楚.

研究的目的:

阐明曼诺2 - 经皮酶 (ME) 的基质识别和催化机制.
确定ME单糖特异性表皮化结构的基础.

主要方法:

使用X射线晶体学来确定Runella slithyformis ME (RsME) 和其D254A突变体在apo和D-glucitol结合状态中的结构.
进行了突变者的动力分析和对接计算.
进行了与其他2 - epimerases的结构比较.

主要成果:

RsME有一个独特的循环 (循环α7-α8),它覆盖了活性口袋,移动以结合D-glucitol.
循环α7-α8中的保存残留物Trp251和Asp254对于D-葡萄糖醇的相互作用和活性至关重要.
Asp254对于连接体结合形状和活性口袋闭合至关重要.
在RSME中,较长的α7-α8循环在硬质上阻碍了二糖体结合,解释了单糖体的特异性.

结论:

已经提出了通过RSME进行基质识别和单糖特异性表皮化的一种详细机制.
独特的循环结构和保存的残留物是ME的功能和特异性的关键.

关键词:

鲁内拉的滑性形状是晶体结构晶体结构酶机制的酶机制是什么曼诺糖2-胺酶是一种基质特异性基质特异性

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