Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

3.9K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
3.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Morphologically Guided Mesoscopic Pore System for Enhancing the Impact Resistance of Stomatopod Telson.

Chem & bio engineering·2026
Same author

Identification and biochemical characterization of a novel porcine circovirus associated with porcine respiratory and diarrheal diseases.

Microbiology spectrum·2025
Same author

A cardioviral 2C-ATP complex structure reveals the essential role of a conserved arginine in regulation of cardioviral 2C activity.

Journal of virology·2024
Same author

StreamSAXS: a Python-based workflow platform for processing streaming SAXS/WAXS data.

Journal of synchrotron radiation·2024
Same author

Mango wiggler as a novel insertion device providing a large and symmetrical imaging field of view.

Journal of synchrotron radiation·2024
Same author

A step towards 6D WAXD tensor tomography.

IUCrJ·2024
Same journal

Scotty: lattice coincidences in the Protein Data Bank.

Acta crystallographica. Section D, Structural biology·2026
Same journal

Scotty: lattice coincidences for macromolecular crystallographic phasing.

Acta crystallographica. Section D, Structural biology·2026
Same journal

Miroslav Z. Papiz (1955-2026).

Acta crystallographica. Section D, Structural biology·2026
Same journal

Structural basis of regioselective double halogenation of the β-carboline tryptoline by the single-component halogenase AetF.

Acta crystallographica. Section D, Structural biology·2026
Same journal

Simulating neutron protein crystallography experiments: applications to the development of the NMX instrument at ESS.

Acta crystallographica. Section D, Structural biology·2026
Same journal

Molecular architecture of the human citrate synthase-malate dehydrogenase 2 metabolon.

Acta crystallographica. Section D, Structural biology·2026
查看所有相关文章

相关实验视频

Updated: Jul 26, 2025

Microcrystallography of Protein Crystals and In Cellulo Diffraction
09:35

Microcrystallography of Protein Crystals and In Cellulo Diffraction

Published on: July 21, 2017

9.1K

用低分辨率衍射数据对具有高溶剂含量的蛋白质晶体进行直接相位算法.

Yu Meng Jiang1, Han Miao1, Xin Yu Pan1

  • 1Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, People's Republic of China.

Acta crystallographica. Section D, Structural biology
|June 14, 2023
PubMed
概括
此摘要是机器生成的。

这项研究引入了蛋白质结晶学阶段检索的新方法,消除了对先前结构知识的需求. 这种相位算法的进步为解决蛋白质相位问题提供了更容易获得的方法.

关键词:
代投影算法 代投影算法低分辨率的信封封面.阶段确定阶段的确定.阶段化分阶段化.

更多相关视频

Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD
13:34

Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD

Published on: December 30, 2016

11.6K
Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography
11:48

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography

Published on: April 24, 2018

14.8K

相关实验视频

Last Updated: Jul 26, 2025

Microcrystallography of Protein Crystals and In Cellulo Diffraction
09:35

Microcrystallography of Protein Crystals and In Cellulo Diffraction

Published on: July 21, 2017

9.1K
Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD
13:34

Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD

Published on: December 30, 2016

11.6K
Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography
11:48

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography

Published on: April 24, 2018

14.8K

科学领域:

  • 晶体学 晶体学是指结晶学.
  • 结构生物学 结构生物学
  • 生物物理学的生物物理.

背景情况:

  • 代投影算法是解决晶体学中蛋白质相位问题的关键.
  • 以前的方法需要先前的知识 (例如,低分辨率的信封),限制了他们的应用.

研究的目的:

  • 开发一种新的阶段检索工作流程,绕过对参考密度分布的需求.
  • 仅使用低分辨率衍射数据来实现直接相位检索.

主要方法:

  • 一个新的工作流使用低分辨率的衍射数据进行相位检索.
  • 最初的阶段分配是随机生成的,并以代方式改进.
  • 在每个阶段检索运行后,应用密度修改.
  • 信息被引入为衡量阶段检索成功的指标.

主要成果:

  • 新的工作流成功地检索了没有先前结构信息的阶段.
  • 在十个高溶剂含量蛋白质结构的验证中,证明了强度.
  • 信息在评估阶段检索结果方面被证明是有效的.

结论:

  • 这种方法克服了先前阶段检索技术的局限性.
  • 它提供了一种更直接,更广泛的方法来解决蛋白质阶段问题.
  • 使用低分辨率数据和信息增强了分阶段处理能力.