Circ_0091579 通过miR-1270/YAP1轴阻断抑制HCC扩散和谷氨酸代谢
Ming Liu1, Bing Guo1, Ge Zhang1
1Department of Radiotherapy, Taian City Central Hospital, No. 29, Longtan Road, Taishan District, Tai'an, Shandong, China.
Biochemical genetics
|June 14, 2023
概括
循环RNAs (circRNAs) 促进肝细胞癌 (HCC) 的进展. 这项研究发现hsa_circ_0091579通过海绵miR-1270促进HCC,影响miR-1270/YAP1轴,提供潜在的HCC生物标志物和治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 循环RNAs (circRNAs) 越来越多地被认为是它们在恶性瘤进展中的作用.
- 肝细胞癌 (HCC) 是一个重要的全球健康问题,需要研究其潜在的分子机制.
- 特定circRNAs的异常表达已与HCC病变发生有关.
研究的目的:
- 为了研究hsa_circ_0091579 (circ_0091579) 在HCC中的表达模式.
- 为了阐明circ_0091579在HCC进展中的功能性作用.
- 探索HCC中涉及circ_0091579,miR-1270和Yes相关蛋白 (YAP1) 的分子机制.
主要方法:
- 定量实时聚合酶连锁反应 (qRT-PCR) 来评估mRNA水平.
- 细胞活力,细胞亡,入侵和迁移试验 (CCK-8,流细胞计,Transwell,伤口愈合).
- 在体内异种移植瘤测定,双露西法酶记者测定和西斑分析.
主要成果:
- 在HCC组织和细胞中,circ_0091579被显著上调.
- Knockdown of circ_0091579抑制了HCC细胞增殖,诱导了亡,并在体内抑制了瘤生长.
- circ_0091579作为miR-1270的分子海绵,YAP1是miR-1270的目标,形成circ_0091579/miR-1270/YAP1调节轴.
结论:
- 通过调节miR-1270/YAP1轴,circ_0091579促进了HCC的进展.
- circ_0091579可以作为HCC的潜在诊断生物标志物.
- 向circ_0091579为HCC治疗提供了一个有前途的治疗策略.
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