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相关概念视频

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

5.9K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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相关实验视频

Updated: Jul 26, 2025

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
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Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Published on: May 3, 2022

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总内部反射显微镜:一种强大的工具,用于探索近接口的相互作用和动态.

Jiahao Wu1, Wei Liu2, To Ngai1

  • 1Department of Chemistry, The Chinese University of Hong Kong, N.T., Shatin, Hong Kong, China. weiliu@jiangnan.edu.cn.

Soft matter
|June 14, 2023
PubMed
概括

总内部反射显微镜 (TIRM) 是一种超敏感的,非侵入性的技术,用于研究在接口附近的相互作用. 这份审查详细介绍了TIRM的期限.

更多相关视频

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy
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Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

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Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules
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Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

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相关实验视频

Last Updated: Jul 26, 2025

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
06:43

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Published on: May 3, 2022

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Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy
10:19

Visualizing Adhesion Formation in Cells by Means of Advanced Spinning Disk-Total Internal Reflection Fluorescence Microscopy

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Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules
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Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

Published on: August 8, 2019

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科学领域:

  • 表面科学是一门科学.
  • 显微镜技术的使用方法
  • 物理化学 物理化学

背景情况:

  • 接近接口的相互作用和动态对于微观/宏观现象至关重要.
  • 描述近接口现象需要先进的分析工具.

研究的目的:

  • 审查总内部反射显微镜 (TIRM) 的原则,应用和最近的发展.
  • 突出TIRM在研究界面动态方面的进展和潜力.

主要方法:

  • 介绍TIRM的原则和特点.
  • 对TIRM的典型测量和最近的技术进步进行了审查.

主要成果:

  • TIRM是一种超敏感且非侵入性的近接口分析技术.
  • 几十年来TIRM的能力和应用的详细审查.

结论:

  • 在描述界面交互和动态方面,TIRM已经取得了重大进展.
  • 在各种科学领域,TIRM具有广泛影响的巨大潜力.