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乳腺癌细胞中介细胞过渡和转移是由DAP5/eIF3d介导的选择性mRNA翻译指导的转移和转移

Amandine Alard¹, Olga Katsara¹, Tiffany Rios-Fuller¹

¹Department of Microbiology, NYU School of Medicine, New York, NY 10016, USA.

|June 14, 2023

概括

一个涉及DAP5/eIF3d的新型mRNA转化途径对于癌症转移和上皮转移至介质细胞转移 (EMT) 至关重要. 这一途径选择性地转化关键基因,为晚期癌症提供新的治疗点.

科学领域:

分子生物学分子生物学
癌症研究癌症研究
翻译医学是一种翻译医学.

背景情况:

癌细胞的可塑性允许通过诸如上皮细胞转移到介质细胞转移 (EMT) 等过程的生存和转移.
标准上限依赖的mRNA翻译涉及eIF4E/mTORC1,但癌症中可能存在其他机制.
了解这些机制是针对癌症入侵和转移的关键.

研究的目的:

调查癌症进展中mRNA翻译的另类机制.
确定DAP5/eIF3d复合体在癌症转移,EMT和血管生成中的作用.
探索DAP5作为转移性乳腺癌的潜在治疗点.

主要方法:

进行了全基因组的转录组和转录组分析.
研究使用了人类和小鼠乳腺癌模型.
研究了DAP5/eIF3d在EMT,细胞迁移,入侵,转移和血管生成中的作用.

主要成果:

DAP5/eIF3d复合体选择性地翻译编码EMT因子,迁移蛋白和血管生成调节者的mRNA.
在转移性乳腺癌中,DAP5过度表达,与无转移性生存率差相关.
DAP5对于转移,EMT和血管生成至关重要,但在癌症模型中并非主要瘤生长.

结论:

癌细胞利用两个不同的mRNA翻译途径:eIF4E/mTORC1和DAP5/eIF3d.
DAP5/eIF3d通路对于癌细胞的可塑性,入侵,转移和血管生成至关重要.
这些发现揭示了癌症进展期间显著的mRNA翻译可塑性,并突出了DAP5作为潜在的治疗点.

关键词:

CP: 癌症癌症癌症科普:分子生物学分子生物学 DAP5 DAP5 的意思是DAP5 的意思.乳腺癌乳腺癌乳腺癌表皮细胞到介质细胞的过渡.转移转移转移转移蛋白质合成蛋白质合成开始翻译开始翻译

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