生理学和转录组分析显示,通过调节抗氧化过程和光合作用,-促进应激下大米苗的发展
Yao Li1,2,3, Hang Zhou1,3, Naijie Feng1,3,4
1College of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang, Guangdong, China.
PloS one
|June 14, 2023
概括
益二 (Pro-Ca) 通过增强抗氧化活性和光合作用来增强米苗对盐应激的抵抗力. 这种植物生长调节剂提高了净光合作用率,减少了离子积累,有助于在盐水条件下生存.
科学领域:
- 植物科学 植物科学
- 农业科学 农业科学
- 压力生理学 压力生理学
背景情况:
- 无生物应激,特别是盐应激,严重阻碍了全球的作物生产率.
- 众所周知,prohexadione-calcium (Pro-Ca) 可以减轻各种植物非生物压力.
- 通过Pro-Ca减轻大米盐应激的具体机制仍未得到充分研究.
研究的目的:
- 为了研究外源性Pro-Ca对面临盐应激的苗的保护作用.
- 阐明米中Pro-Ca介导盐耐受性的潜在生理和分子机制.
主要方法:
- 米苗被用50 mmol·L-1 NaCl溶液处理,含有或没有100 mg·L-1 Pro-Ca.
- 分析了与抗氧化酶和光合作用相关的基因表达.
- 测量了酶活性 (酸盐过氧化酶,超氧化物脱氧化酶,过氧化酶),甲水平,净光合作用速率和Na+度.
主要成果:
- 在盐应力下,Pro-Ca的应用显著增加了酸盐过氧化酶 (84.2%),超氧化脱氧化酶 (75.2%) 和过氧化酶 (3.5%) 的活性.
- 随着Pro-Ca治疗,马隆迪阿尔海德水平降低了5.8%.
- 净光合作用率增加了167.2%,与盐压力对照相比,Pro-Ca处理的苗木中Na+度降低了17.1%.
- 普罗卡调节了关键抗氧化剂 (SOD2,PXMP2,MPV17,E1.11.1.7) 和光合作用/叶绿素代谢基因 (PsbS,PsbD,heml,PPD) 的表达.
结论:
- 普罗赫萨迪-有效地提高了水苗的盐分耐受性.
- Pro-Ca通过调节抗氧化剂防御系统和提高光合作用效率来起作用.
- 这些发现突出了Pro-Ca作为改善盐水环境中米种植的潜在剂.
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