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克莱德-D助氨酸反应因子调节助氨酸信号传递和发育在的Physcomitrium patens

Carlisle Bascom¹, Michael J Prigge¹, Whitnie Szutu¹

¹Department of Cell and Developmental Biology, School of Biological Sciences, University of California San Diego, San Diego, California, United States of America.

|June 14, 2023

概括

克莱德-D辅酶反应因子 (ARFs) 是菌体Physcomitrium patens中的转录激活剂,影响发育. 这些ARF通过与DNA结合的clade-AARF相互作用来增强基因表达.

科学领域:

植物分子生物学植物分子生物学
发展生物学发展生物学
转录条例转录条例转录条例

背景情况:

辅酶响应因子 (ARF) 调节基因表达以应对辅酶.
ARF被分为激活剂和抑制剂.
在白植物和白植物中发现的Clade-D ARF缺乏DNA结合域,它们的功能尚不清楚.

研究的目的:

在模型 bryophyte Physcomitrium patens 中研究 clade-D ARF 的转录活性.
确定D类ARF在植物发育中的作用.
阐明 clade-D ARF 调节基因表达的机制.

主要方法:

对缺乏clade-D ARFs (Δarfddub) 的Physcomitrium patens突变体的遗传分析.
观察发育现象型,包括丝分支和细胞类型转换.
蛋白相互作用研究以确定D类ARFs的相互作用伙伴.
对ARFd1活性进行寡合化试验.

主要成果:

克莱德-D ARFs 在 Physcomitrium patens 中作为转录激活剂起作用.
关节-D ARFs (Δarfddub) 的损失导致了延迟的线丝分支和色膜向色膜的过渡.
在Darfddub线中,叶子性相体的发育也被延迟了.
ARFd1通过PB1域与激活ARF相互作用,而不是抑制ARF.
ARFd1需要寡合化才能获得完全的转录活性.

结论:

克莱德-D ARF 是基因表达的重要激活剂,在植物发育中起着重要作用.
一个拟议的模型表明,clade-D ARFs通过与DNA结合的clade-A ARFs相互作用来增强基因表达.
类D ARFs的寡合化对于它们在转录激活中的功能至关重要.

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