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工程生物组织的自我氧化调节了介质细胞干细胞的骨质性承诺

Shabir Hassan¹, Ting Wang², Kun Shi³

¹Division of Engineering in Medicine, Department of Medicine, Harvard Medical School, and Brigham and Women's Hospital, Cambridge, MA, 02139, USA; Department of Biology, College of Arts and Sciences, Khalifa University (Main Campus), Abu Dhabi, P.O. Box, 127788, United Arab Emirates; Advanced Materials Chemistry Center (AMCC), Khalifa University (SAN Campus), Abu Dhabi, P.O. Box, 127788, United Arab Emirates.

|June 14, 2023

概括

产生氧气的微粒子改善了即使在低氧条件下,在水凝中的骨细胞 (hMSC) 分化. 这增强了用于再生医学的工程组织形成.

科学领域:

生物材料科学生物材料科学
组织工程是组织工程.
细胞生物学细胞生物学

背景情况:

氧化生物材料可以减轻无氧压力,并增强植入物集成.
释放氧气的材料对组织形成的影响,特别是骨质生成,需要进一步研究.

研究的目的:

研究基于过氧化 (CPO) 的产生氧气的微粒子 (OMPs) 对人类介质干细胞 (hMSC) 骨质分化的作用.
为了比较水凝与OMP,酸盐纳米颗粒 (SNPs) 或在正常和无氧条件下两者的骨质生成潜力.

主要方法:

在polycaprolactone中微封装CPO,以创建用于持续释放氧气的OMP.
制造含有OMP,SNP或两者的凝甲基烯基 (GelMA) 水凝.
使用批量mRNAseq和时间依赖因子表达分析评估hMSC骨质分化.
评估皮下植入对宿主细胞入侵和血管生成的影响.

主要成果:

含有OMP的水凝在正常和无氧条件下促进了hMSC的骨质分化.
与SNP或SNP/OMP水凝相比,无氧OMP水凝显示出更强的骨质生路径调节.
皮下植入物显示SNP水凝中宿主细胞入侵和血管生成增加.

结论:

生氧微粒可以诱导,改善和指导功能性工程生物组织的形成.
这种方法对组织再生和器官替代疗法的应用具有重大前景.
水凝氧化是一种可行的策略,可以在具有挑战性的微环境中增强骨质分化和组织发育.

关键词:

骨形成骨的形成骨质发生过程 (osteogenesis)氧化材料是一种氧化材料.干细胞的命运干细胞的命运组织工程是组织工程.

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相关概念视频

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...