在单极细胞运动过程中,v-Src通过抑制Aurora B激酶活性来移位Aurora B
Ryoko Ota1, Takumi Watanabe1, Yuuki Wazawa1
1Laboratory of Biochemistry and Molecular Biology, Kyoto Pharmaceutical University, Kyoto 607-8414, Japan.
Cellular signalling
|June 14, 2023
概括
致癌性v-Src蛋白质会导致Aurora B移位,导致细胞分裂错误. 这项研究揭示了v-Src间接抑制了Aurora B激酶活性,从而导致癌症的进展.
科学领域:
- 细胞生物学 细胞生物学
- 分子瘤学分子瘤学
- 癌症研究 癌症研究
背景情况:
- c-Src氨酸激酶在细胞信号传递中至关重要,其活性升高与各种癌症有关.
- v-Src是一种c-Src的致癌形式,表现出构成性氨酸激酶活性,并且已知会破坏细胞分裂.
研究的目的:
- 为了阐明v-Src诱导光环B移位的机制.
- 为了研究v-Src对Aurora B激酶活性和细胞分裂期间局部化的影响.
主要方法:
- 用EG5抑制剂 (STLC) 和循环素依赖性激酶1 (CDK1) 抑制剂 (RO-3306) 治疗细胞,以诱导单极细胞运动.
- 诱导v-Src表达,并观察到Aurora B的局部化.
- 进行了西部涂抹和体外激酶试验,以评估Aurora B自酸化和激酶活性.
- 细胞还被用Aurora B抑制剂 (ZM447439) 治疗,以进行比较.
主要成果:
- v-Src表达诱导了在经历单极细胞运动的细胞中Aurora B移位.
- 发现v-Src降低了Aurora B自酸化和整体酶活性.
- 由 ZM447439 抑制 Aurora B 激酶活动模仿了 v-Src 诱导的移位.
- v-Src并没有直接化Aurora B.
结论:
- v-Src间接地抑制了Aurora B激酶活性,导致其脱局部.
- 这种机制有助于细胞运动失败和在v-Src表达细胞中观察到的双核细胞形成.
- 了解这种途径可能为具有高Src活性的癌症提供治疗点.
关键词:
在AZ3146中,它是AZ3146.极光 B 极光 B 极光在CDK1中,CDK1是指CDK1.细胞动力学 细胞动力学单极细胞运动的单极细胞运动.Mps1 是一个Mps1.这里是RO-3306的位置.在STLC中,STLC是STLC.这就是V-Srccc.更多相关视频
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