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AxonQuantifier:一个半自动程序,用于从整体安装的视神经中量化轴突密度.

Micalla G Peng1, Jonathan Lee1, Wilson Ho1

  • 1Department of Ophthalmology, Keck School of Medicine, USC Roski Eye Institute, University of Southern California, Los Angeles, CA, USA.

Journal of neuroscience methods
|June 14, 2023
PubMed
概括

一种新的半自动化方法,AxonQuantifier,有效地量化了整个视神经中的视网膜质细胞 (RGC) 轴突密度. 与手动RGC轴子密度测量相比,该工具提高了准确性和速度.

关键词:
亚克逊人是一个亚克逊人.神经再生是一种神经再生.神经保护是一种神经保护.眼神经的视觉神经是什么量化 量化 量化 量化视网膜的质细胞细胞.

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科学领域:

  • 神经科学是一个神经科学.
  • 眼科医生 眼科 眼科
  • 生物医学成像技术 生物医学成像技术

背景情况:

  • 视网膜质细胞 (RGC) 轴突密度量化对于理解视神经损伤和再生至关重要.
  • 现有的手动方法耗时,容易导致评价者之间的变化.

研究的目的:

  • 提交和验证AxonQuantifier,这是一个半自动化方法来量化RGC轴突密度.
  • 将AxonQuantifier的效率和准确性与手动量化进行比较.

主要方法:

  • 开发了一个半自动算法,AxonQuantifier,运行在ImageJ软件上.
  • 使用纵向共聚焦显微镜对整个安装的老鼠视神经损伤后的图像.
  • 量化RGC轴突密度在远距离到视神经压碎部位的不同距离.

主要成果:

  • 与手动量化相比,AxonQuantifier的时间效率提高了4倍.
  • 半自动化方法显示,提高了评价者之间的一致性,减少了偏差.
  • 与手动评估相比,AxonQuantifier倾向于略微低估轴突密度.

结论:

  • AxonQuantifier是一种可靠和高效的工具,用于评估整体安装视神经制剂中的RGC轴突密度.
  • 这种方法比手工量化技术有了显著的进步.