衰老和损伤会影响肌的核形状异质性
Yann Tinguely1,2, Vivian Shi1,2, Franka Klatte-Schulz3,4
1John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA.
概括
肌组织中的细胞核形状变化与衰老和损伤有关. 新的图像分析方法揭示了特定区域的独特核形态,有助于理解组织变化.
科学领域:
- 生物医学工程 生物医学工程
- 组织工程是组织工程.
- 细胞生物学 细胞生物学
背景情况:
- 带有定性评分的组织学斑点是组织分析的标准,但缺乏灵敏度,无法捕捉细胞异质性.
- 定量分析提供了对病理机制的洞察力,但不能完全代表细胞亚群之间的结构变异.
- 细胞和核的形状越来越被认为是细胞功能和功能障碍的关键指标.
研究的目的:
- 开发和应用先进的图像分析技术,用于定量评估肌组织中的细胞和核形态.
- 研究细胞核形状,衰老和肌损伤之间的关联.
- 在特定的细胞外矩阵区域内探索细胞核群体的异质性.
主要方法:
- 利用视觉辅助形态表型图像识别 (VAMPIRE) 来基于形状的自动细胞细分.
- 集成的VAMPIRE具有在蛋白质丰富的细胞外基质区域区分细胞的能力.
- 将该方法应用于老化和受伤的老鼠和人类肌组织.
主要成果:
- 在老鼠肌中识别了成熟和衰老期间的多个核形状模式.
- 在衰老过程中发现了蛋白质甘氨酸丰富区域中细胞核形状的独特子组.
- 观察到与老鼠肌损伤相关的免疫标志物 (αSMA,CD31,CD146) 与更圆的核形状相关.
- 发现受伤部位的人体肌细胞核与未受伤组织相比更圆.
结论:
- 在衰老和受伤期间肌组织的变化与细胞核形态的变化有关.
- 该研究根据细胞核形状确定了特定区域的细胞子群.
- 开发的方法提高了对肌衰老和损伤中的细胞异质性的理解,并为临床应用提供了潜力.
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