Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jul 26, 2025

A Silicon-tipped Fiber-optic Sensing Platform with High Resolution and Fast Response
09:03

A Silicon-tipped Fiber-optic Sensing Platform with High Resolution and Fast Response

Published on: January 7, 2019

7.2K

对于可部署的传感应用程序,快速和精细调整的表达方式.

Alexandra T Patterson1, Mark P Styczynski2

  • 1School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, USA.

Advances in biochemical engineering/biotechnology
|June 14, 2023
PubMed
概括

无细胞表达系统为开发可在现场部署的生物传感器提供了一种经济有效且快速的替代方案. 通过仔细的工程来优化这些系统,提高了它们的灵敏度和分子检测效率.

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Harnessing Plasmid Crosstalk in Transcription Factor Mediated Cell-Free Biosensors.

bioRxiv : the preprint server for biology·2025
Same author

A mathematical model of cell-free transcription-translation with plasmid crosstalk.

Synthetic biology (Oxford, England)·2025
Same author

Copper nanocubes as low-cost enzyme mimics in a sarcosine-sensing reaction cascade.

The Analyst·2025
Same author

A modular cell-free protein biosensor platform using split T7 RNA polymerase.

Science advances·2025
Same author

Assessing structural uncertainty of biochemical regulatory networks in metabolic pathways under varying data quality.

NPJ systems biology and applications·2024
Same author

A modular cell-free protein biosensor platform using split T7 RNA polymerase.

bioRxiv : the preprint server for biology·2024

科学领域:

  • 生物技术是生物技术.
  • 生物传感器开发开发
  • 分子生物学分子生物学

背景情况:

  • 有机体拥有高效的分子传感机器,对生物传感器的开发有价值.
  • 净化用于体外生物传感器的生物分子机械是昂贵的.
  • 全细胞体内生物传感器的响应时间缓慢,样本敏感性问题.

研究的目的:

  • 为应对现场部署生物传感器的无细胞蛋白质表达系统的实施挑战.
  • 为严格的生物传感器标准优化无细胞系统.
  • 为了实现快速的传感器读取和经济高效的生产.

主要方法:

  • 专注于实施无细胞蛋白质表达系统.
  • 通过选择传感器和输出元件来微调表达.
  • 优化反应条件:DNA/RNA度,溶解物制备,缓冲条件.

主要成果:

  • 无细胞系统克服了纯化机械和全细胞生物传感器的局限性.
  • 允许在有毒环境中增加功能和快速传感器读取.
  • 与净化相比,实现了合理的生产成本.

结论:

关键词:
生物传感器是一种生物传感器.细胞自由表达的表达.遗传的电路是基因的.软化液化化是什么意思快速表达方式 快速表达方式

更多相关视频

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

9.3K
Production of a Strain-Measuring Device with an Improved 3D Printer
06:17

Production of a Strain-Measuring Device with an Improved 3D Printer

Published on: January 30, 2020

6.2K

相关实验视频

Last Updated: Jul 26, 2025

A Silicon-tipped Fiber-optic Sensing Platform with High Resolution and Fast Response
09:03

A Silicon-tipped Fiber-optic Sensing Platform with High Resolution and Fast Response

Published on: January 7, 2019

7.2K
Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

9.3K
Production of a Strain-Measuring Device with an Improved 3D Printer
06:17

Production of a Strain-Measuring Device with an Improved 3D Printer

Published on: January 30, 2020

6.2K
  • 无细胞系统为现场部署的生物传感器提供了一个可行的平台.
  • 仔细的传感器工程和优化是成功的关键.
  • 能够产生严格调节的,快速表达的生物传感器遗传电路.