骨关节炎性软骨细胞在暴露于IL-1b或TNF时经历了与糖解相关的代谢切换
Anais Defois1, Nina Bon1, Alexandre Charpentier1
1Nantes Université, Oniris, CHU Nantes, INSERM, Regenerative Medicine and Skeleton, RMeS, UMR 1229, 44000, Nantes, France.
Cell communication and signaling : CCS
|June 14, 2023
概括
骨关节炎涉及炎症和肌肉细胞的代谢变化. 针对炎症和新陈代谢可能提供新的骨关节炎治疗策略.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 骨关节炎 (OA) 是一种与年龄相关的退行性关节疾病,症状治疗有限.
- 由IL-1b,TNF和IL-6等细胞因子驱动的炎症是OA进展的核心.
- 目前的抗细胞因子疗法表现出有限的成功,这表明了解细胞因子对冠状细胞的影响存在差距.
研究的目的:
- 综合分析OA冠状细胞的转录组和蛋白组概况,这些冠状细胞被促炎性细胞因子刺激.
- 为了将这些形状与非OA冠状细胞的形状进行比较.
- 用细胞代谢测试来功能验证分子失调.
主要方法:
- 用IL-1b,TNF和IL-6治疗的OA和非OA冠状细胞的转录和蛋白质组数据集的生成.
- 对基因表达特征进行比较分析.
- 实时细胞代谢测试以确认功能变化.
主要成果:
- 特定于OA冠状细胞中的失调代谢基因的识别.
- 在接受IL-1b或TNF治疗的OA软体细胞中,证实了代谢转向增加糖解和减少线粒体呼吸的转变.
- 在非OA冠状细胞中没有观察到类似的代谢变化.
结论:
- 在炎症和改变的代谢之间存在强烈的,特定的关联在OA胆固醇细胞中.
- 这种炎症 - 代谢联系在OA冠状细胞中与非OA冠状细胞相比似乎加剧了.
- 研究结果表明,针对炎症和代谢失调之间的相互作用可能是OA的有希望的治疗策略.
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