一个新的3-miRNA网络调节了口腔状细胞癌中的瘤进展
Aditi Patel1, Parina Patel1, Dushyant Mandlik2
1Biological and Life Sciences, School of Arts and Sciences, Ahmedabad University, Ahmedabad, 380009, Gujarat, India.
Biomarker research
|June 14, 2023
概括
这项研究确定了唾液中的3-microRNA (miRNA) 签名,用于早期检测口腔状细胞癌 (OSCC). 这种唾液生物标志物可以预测疾病的进展,并揭示OSCC发展的潜在分子机制.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物标志物发现发现
背景情况:
- 晚期诊断是口腔状细胞癌 (OSCC) 的一个主要挑战,需要早期检测方法.
- 目前的分子诊断缺乏临床上可用的,针对疾病的生物标志物,用于早期的OSCC风险预测.
- 非侵入性液体活检技术对于识别早期口腔癌诊断的生物标志物至关重要.
研究的目的:
- 为了确定唾液外基因组衍生的微RNA (miRNA) 生物标志物,用于早期的OSCC检测.
- 阐明关键的miRNA-mRNA网络和驱动OSCC进展的潜在机制.
- 为了验证已识别的miRNA签名的诊断和预后潜力.
主要方法:
- 在OSCC患者的组织和唾液外体上进行小RNA测序 (小RNASeq) (n=23).
- 癌症基因组图谱 (TCGA) 数据集 (n=114) 和qPCR验证 (n=70) 的综合分析.
- 在OECM-1细胞系中进行miRNA-mRNA网络分析,通路分析和功能测试.
主要成果:
- 一个3-miRNA签名 (miR-140-5p,miR-143-5p,miR-145-5p) 在OSCC中被确定为显著下调.
- 这种特征有效地预测了疾病的进展,并与不良预后相关 (p<0.05).
- 在试验室中对3-miRNA签名的升级减少了增殖,诱导了细胞亡,导致细胞循环停止,并通过逆转上皮质-介质细胞转换 (EMT) 来抑制侵入/迁移.
结论:
- 唾液外体体中的3-miRNA签名显示了作为OSCC进展的非侵入性生物标志物的潜力.
- 已识别的miRNA及其相关网络为OSCC发展的分子机制提供了洞察力.
- 这一发现促进了口腔状细胞癌的早期检测和风险分层.
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